骨痹颗粒质量标准研究  

Study for Quality Standard of Gubi Granules

作　　者：薛世姣 谈聪[1,3] 马世平[2] 陆瑾[1,3] 李博[1,3] 潘林梅[1,3] 郭立玮[1,3] 朱华旭[1,3] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]中国药科大学中药学院,江苏南京211198 [3]南京中医药大学江苏省植物药深加工工程研究中心,江苏南京210029

出　　处：《中药新药与临床药理》2014年第5期614-618,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省科技支撑计划--社会发展项目(BE2011847);产学研联合创新资金--前瞻性联合研究项目(BY2012036)

摘　　要：目的建立骨痹颗粒的质量标准。方法采用薄层色谱（TLC）法对该制剂中桑寄生、续断、骨碎补进行定性鉴别;采用高效液相色谱法测定该制剂中续断的有效成分川续断皂苷Ⅵ的含量。结果桑寄生、续断、骨碎补的TLC别专属性强;川续断皂苷Ⅵ在0.24～15.33μg范围内呈良好的线性关系,平均回收率为98.36%,RSD为1.09%（n=9）。结论该定性、定量检测方法简便可行、专属性强、重现性好、灵敏度高,可作为骨痹颗粒的质量控制标准。Objective To establish the quality standard for Gubi Granules. Methods Herba Taxilli, Radix Dipsaci, Rhizoma Drynariae in Gubi Granules were identified by thin layer chromatography （TLC）, and the content of asperosaponin VI of Radix Dipsaci was determined by high performance liquid chromatography（HPLC）. Results TLC could identify Herba Taxilli, Radix Dipsaci, Rhizoma Drynariae effectively. A good linearity of asperosaponin VI was in the range of 0.24 - 15.33μg. The average recovery was 98.36 %, and RSD was 1.09 %（n=9）. Conclusion The established methods are simple, specific, reproducible, and sensitive, and they can be used for the quality control of Gubi Granules.

关 键 词：骨痹颗粒 薄层色谱法 高效液相色谱法 川续断皂苷Ⅵ 质量标准 

分 类 号：R284.1[医药卫生—中药学]