内毒素腹腔注射法制备小鼠急性肺损伤肺纤维化模型及中药干预研究  

Preparation of mouse acute pulmonary fibrosis mode by endotoxin intraperitoneal injection and intervention research of Chinese Medicine

作　　者：贾先红[1] 朱红梅[1] 吴瑕[2] 吕俊秀[1] 郝传传[1] 

机构地区：[1]徐州医学院附属医院,江苏徐州221002 [2]徐州医学院病理生理学教研室,江苏徐州221004

出　　处：《中医临床研究》2014年第30期139-141,共3页Clinical Journal Of Chinese Medicine

摘　　要：目的:观察内毒素腹腔注射对小鼠急性肺损伤及肺纤维化的影响及其在中药干预下的变化。方法:10mg/kg内毒素一次性腹腔注射制备小鼠急性肺损伤模型,造模即日起分别给予补肺通络中药或生理盐水灌胃,各组分别于d14、d28处死,观察不同处理条件下肺损伤及肺纤维化随时间的变化情况,HE染色观察肺组织形态学改变,Masson染色观察肺组织纤维化程度。结果:1d14时,给药组先于模型组进入损伤修复阶段,其肺组织纤维化评分(13.17±6.68)显著高于单纯模型组(2.38±1.06)(P<0.01),而肺损伤评分给药组(1.88±0.45)与模型组(1.70±0.31)尚无明显差别(P>0.05);2d28时,模型组纤维化水平有较大幅度增加,而给药组变化则相对较小,模型组纤维化评分(18.44±2.65)与给药组(17.71±3.25)近似,二者间无统计学差异;两组小鼠肺组织炎症反应均有所消退,给药组基本接近正常而模型组尚存在较明显的炎症反应,肺损伤评分给药组(0.49±0.14)低于模型组(0.82±0.14)(P<0.01)。结论:内毒素能够替代博来霉素用以制备小鼠急性肺损伤肺纤维化模型。Objective： To observe the impacts of endotoxin intraperitoneal injection on preparation of mouse acute pulmonaryfibrosis mode and its changes under the intervention of Chinese medicine. Methods： Acute lung injury model of mice by intraperitonealinjection of 10mg/Kg lipopolysaccharide preparation, medicines on tonifying lung and activating meridians or saline were given to micefrom the 1st day, rats in each group were killed on D14, D28. The different conditions of lung injury and pulmonary fibrosis were observed.the morphological changes were observed by lung tissue HE staining, and the degree of lung tissue fibrosis was observed by Massonstaining . Results： On D14, the medicine group entered the damage repair phase earlier than the model group, its pulmonary fibrosis score（13.17±6.68） was significantly higher than that in the model group （2.38±1.06） （P〈0.01）, and the lung injury scores of the medicinegroup （1.88±0.45） and the model group （1.70±0.31） had no significantly difference （P〉0.05）; On D28, the level of fibrosis model groupincreased significantly, while the changes of the medicine group was relatively small, the fibrosis score of the model group （18.44±2.65）and medication group （17.71±3.25） were approximated with no statistical difference; the lung tissue inflammation of the two groups ofmice had relived, the meditation group was basically close to the normal and the model groups still had obvious inflammatory reaction andlung injury score of the meditation group （0.49±0.14） was lower than that of the model group （0.82±0.14） （P〈0.01）. Conclusions：Endotoxin can replace bleomycin for the preparation of acute lung injury in mice model of pulmonary fibrosis.

关 键 词：内毒素 肺纤维化 博来霉素 补肺通络 小鼠 

分 类 号：R256.1[医药卫生—中医内科学]