下调TSPO表达不影响脂多糖诱导的小胶质细胞TNF-α和IL-1β以及IL-6的分泌  

Down-regulation of TSPO expression doesn't affect the productions of TNF-α,IL-1β and IL-6 in LPS-stimulated BV-2 microglia

作　　者：豆晓锋[1] 张亚飞[1] 陈强[2] 李文娇[2] 李洋[2] 俞春东[2] 苏新辉[1] 

机构地区：[1]厦门大学附属第一医院核医学科,福建厦门361003 [2]厦门大学生命科学学院,福建厦门361102

出　　处：《细胞与分子免疫学杂志》2014年第9期897-900,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81071182);福建省医学创新项目(2009-CXB-46);厦门大学生命科学学院细胞生物和肿瘤工程教育部重点实验室开发基金(2009101);福建医科大学非直属附属医院科研发展专项基金(2008031)

摘　　要：目的研究相对分子质量(Mr)18 000转运蛋白(TSPO)基因表达下调对脂多糖(LPS)诱导BV-2小胶质细胞分泌TNF-α,IL-1β和IL-6的影响。方法以RNA干扰技术建立TSPO基因表达下调的细胞模型,实时荧光定量PCR(qRT-PCR)和Western blot法检测转染TSPO siRNA BV-2细胞TSPO mRNA及蛋白水平表达的效果;用qRT-PCR法检测TSPO基因下调后小胶质细胞BV-2在LPS作用下分泌TNF-α、IL-1β和IL-6的mRNA水平表达的情况;ELISA检测TSPO基因下调小胶质细胞BV-2在LPS作用下分泌TNF-α、IL-1β和IL-6的蛋白水平表达的变化。结果成功建立了TSPO基因下调的细胞模型,稳定表达TSPO siRNA细胞的TSPO mRNA和蛋白水平表达均明显下降,TSPO基因下调后BV-2细胞在LPS作用下分泌TNF-α、IL-1β和IL-6的量无变化。结论下调TSPO的表达对LPS刺激引起的小胶质细胞TNF-α、IL-1β和IL-6的分泌无明显影响。Objective To evaluate the effect of translocator protein（ TSPO,Mr18 000） on the productions of TNF-α,IL-1βand IL-6 in lipopolysaccharide（ LPS）-stimulated BV-2 cells. Methods RNA interference technique was used to decrease TSPO expression in BV-2 cells. Western blotting was performed to assess the level of TSPO protein in BV-2 cells transfected with TSPO siRNA. Then the mRNA and protein levels of TNF-α,IL-1β,and IL-6 were measured in LPS-stimulated BV-2 cells by real-time quantitative PCR（ qRT-PCR） and ELISA,respectively. Results The level of TSPO protein obviously decreased in BV-2 cells transfected with TSPO siRNA. However,knockdown of TSPO had no effect on the productions of TNF-α,IL-1βand IL-6 in LPS-stimulated BV-2 cells. Conclusion Down-regulated TSPO is not directly involved in regulating TNF-α,IL-1βand IL-6 productions induced by LPS in microglia.

关 键 词：TSPO基因 RNA干扰 炎症反应 小胶质细胞 

分 类 号：R392.11[医药卫生—免疫学] R392-33[医药卫生—基础医学]