类叶升麻苷增强致亚急性衰老小鼠脑组织神经营养因子3的表达  

Acteoside enhances expression of neurotrophin-3 in brain tissues of subacute aging mice induced by D-galactose combined with aluminum trichloride

作　　者：高莉[1] 彭晓明 霍仕霞 何燕 闫明 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [2]新疆维吾尔自治区维吾尔医药研究所,新疆维吾尔医方剂学重点实验室,新疆乌鲁木齐830049

出　　处：《细胞与分子免疫学杂志》2014年第10期1022-1025,1029,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：新疆维吾尔自治区科研机构创新发展专项资金(2012015);新疆维吾尔自治区公益性科研院所基金项目(KY2013103)

摘　　要：目的观察类叶升麻苷对D-半乳糖联合AlCl3致亚急性衰老模型小鼠脑组织神经营养因子3(NT-3)表达的影响。方法雌性昆明小鼠随机分为溶媒对照组、亚急性衰老模型组、维生素E组、吡拉西坦组和类叶升麻苷治疗组。腹腔注射D-半乳糖60 mg/(kg·d),AlCl35mg/(kg·d)灌胃,连续90 d,建立亚急性衰老小鼠模型。荧光定量PCR检测小鼠脑组织NT-3 mRNA表达水平,免疫组织化学技术和Western blot法检测NT-3的蛋白表达。结果 NT-3在亚急性衰老模型小鼠脑组织中表达明显降低。模型小鼠给予类叶升麻苷、维生素E、吡拉西坦后,脑组织NT-3 mRNA和蛋白表达水平均显著增强。结论类叶升麻苷可以促进亚急性衰老小鼠脑组织中NT-3 mRNA和蛋白表达水平增强。Objective To study the effect of acteoside on the expression of neurotrophin-3 （NT-3） in brain tissues of subacute aging mice induced by D-galactose （D-Gal） combined with aluminum tdchloride （AlCl3 ）. Methods Female Kunming mice were randomly divided into vehicle control group, D-Gal combined with AICI3 group, vitamin E group, piracetam group and acteoside groups [30, 60, 170 mg/（kg · d）]. Mice in D-Gal combined with AlCl3, vitamin E, piracetam and acteoside groups were injected intraperitoneally （i. p. ） with D-Gal I60 mg/（ kg · d）] and AICI3 E5 rag/（ kg · d）] for 90 days to induce the subacute aging models. The real-time quantitative PCR, Western blotting and immunohistochemistry were respectively used to detect the expression of NT-3 mRNA and protein in cerebral cortex and hippocampus of mouse brain. Results D-Gal and AICI3 caused a significant reduction of NT-3 in cerebral cortex and hippocampus. Acteoside, vitamin E and piracetam increased the decreased expression of NT-3 induced by D-Gal and AlCl3, and the difference was statistically significant （P 〈 0.05 or P 〈0. O1 ）. Conclusion Acteoside can up-regulate the expression of brain NT-3 in D-Gal plus AICI3 treated mice.

关 键 词：亚急性衰老 阿尔茨海默病 神经营养因子3 类叶升麻苷 D-半乳糖 

分 类 号：R392.11[医药卫生—免疫学] R392.33[医药卫生—基础医学]