新生儿细菌性肺炎患者外周血自然杀伤细胞的数量变化及其临床意义  

Changes and clinical significance of peripheral blood natural killer cells in neonates with bacterial pneumonia

作　　者：李秋玲[1] 翁开枝[2] 朱玲[3] 梅序桥[4] 许丽萍[2] 林捷华 

机构地区：[1]漳州卫生职业学院护理系,福建漳州363000 [2]福建医科大学附属漳州市医院儿科,福建漳州363000 [3]福建医科大学基础医学院免疫系,福建福州350108 [4]福建医科大学附属漳州市医院检验科,福建漳州363000 [5]福建医科大学附属漳州市医院产科,福建漳州363000

出　　处：《细胞与分子免疫学杂志》2014年第10期1067-1070,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省教育厅科研项目(JB3363);漳州卫生职业学院院本课题项目(XYZ201306)

摘　　要：目的检测新生儿细菌性肺炎患者外周血总自然杀伤细胞(NK细胞)及其各亚群百分率的变化,探讨NK细胞在其发病中的临床意义。方法采用流式细胞术检测38例新生儿细菌性肺炎患者及18例正常新生儿的总NK细胞及其各亚群占外周血总淋巴细胞百分率,住院天数在10 d以内(含10 d)为A组、住院天数10 d以上为B组;入院时外周血白细胞的数量<5.0×109/L或>20.0×109/L者为重度感染组,5.0×109/L<外周血白细胞数量<20.0×109/L者为轻度感染组。结果新生儿细菌性肺炎患者外周血总NK细胞及CD3-CD56negCD16bright百分率低于正常新生儿(P<0.01),但NK细胞CD3-CD56bright CD16neg/dim及CD3-CD56dimCD16bright亚群差异无统计学意义。A组CD3-CD56negCD16bright亚群百分率低于正常新生儿(P<0.01),而总NK细胞及其他亚群则差异无统计学意义。B组总NK细胞及CD3-CD56negCD16bright亚群百分率低于正常新生儿(P<0.01),且B组总NK细胞及各亚群百分率低于A组(P<0.05)。重度感染组总NK细胞及其各亚群百分率明显低于轻度感染组,差异有统计学意义(P<0.05)。结论新生儿细菌性肺炎患者病情越重住院时间越长,总NK细胞及其各亚群百分率越低。Objective To detect the percentage of total natural killer（ NK） cells and its different populations in the peripheral blood from neonates with bacterial pneumonia and discuss the clinical significance of NK cells in the pathogenesis of bacterial pneumonia. Methods Flow cytometry was performed to detect the percentages of NK cells and its subsets in peripheral blood lymphocytes from 38 cases of neonatal bacterial pneumonias and 18 cases of normal neonates. Patients recruited were divided into two groups according to hospitalization days and numbers of peripheral leukocytes： hospitalization days within 10 days（ including 10 days） as group A,and more than 10 days as group B; the number of peripheral blood leukocytes 〈5. 0 × 10^9/L or 〉20. 0 × 10 ^9/L as severe infection group,and 5. 0 × 10^9/L 〈number of peripheral blood leukocytes 〈20. 0 × 10^9/L as mild infection group. Results The percentages of peripheral blood NK cells and CD3-CD56 neg CD16brightsubset in the neonates with bacterial pneumonia were significantly lower than those of the normal newborns（ P 0.01）,but there were no statistically significant differences in CD3-CD56brightCD16neg/dimand CD3-CD56dimCD16 bright subsets. The percentage of CD3-CD56negCD16 brightsubset in group A was significantly lower than that of the normal newborns（ P〈0. 01）,while the percentages of the total NK cells and other subsets had no statistical significance. The neonates with bacterial pneumonia had significantly lower percentages of the total NK cells and CD3-CD56negCD16 brightsubset in group B as compared with the normal neonates（ P〈0. 01）. And the percentages of the total NK cells and its subsets in group B were also lower than those in group A（ P〈 0. 05）. The percentages of NK cells and each subset in severe infection group were significantly lower than those in mild infection group（ P 〈0. 05）. Conclusion To the neonates who suffer from bacterial pneumonia,the more serious and the longer hospital stay,the lower the percenta

关 键 词：新生儿 细菌性肺炎 自然杀伤细胞 

分 类 号：R722.135[医药卫生—儿科] R392.12[医药卫生—临床医学]