Histological Observation of Germ Cell Development and Discovery of Spermatophores in Ovoviviparous Black Rockfish(Sebastes schlegeli Hilgendorf) in Reproductive Season  

作　　者：FENG Junrong LIU Liming JIANG Haibin WANG Maojian DU Rongbin 

机构地区：[1]Ocean College, Yantai University [2]Shandong Marine Fisheries Research Institute

出　　处：《Journal of Ocean University of China》2014年第5期830-836,共7页中国海洋大学学报（英文版）

基　　金：supported financially by Grand Innovating Program of Agriculture Applying Technique in Shandong Province (No. 2008-109);Modern Agricultural Industry Technology System in Shandong Province and the Science and Technology Development Program of Yantai (No. 2013 ZH088)

摘　　要：Black rockfish(Sebastes schlegeli) is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season(October 2011–November 2012) with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.Black rockfish（Sebastes schlegeli） is an important species for culture; however, its reproductive characteristics have not been fully documented. In this study, we investigated the morphology and developmental process of germ cells in this ovoviviparous rockfish in reproductive season（October 2011–November 2012） with histological methods. We found that the gonad of mature fish showed notable seasonal changes in developmental characteristics and morphological structure. The sperm cells matured during a period lasting from October to December, significantly earlier than the oocytes did. A large number of spermatozoa and other cells occurred in testis at different developmental stages. Vitellogenesis in oocytes began in October, and gestation appeared in April next year. Spermatophores were discovered for the first time in Sebastes, which assembled in testis, main sperm duct, oviduct and genital tract, as well as ovarian cavity in October and April. These organs may serve either as production or hiding places for spermatophores and spermatozoa which were stored and transported in form of spermatophores. Testicular degeneration started from the distal part of testis in April, with spermatophores assembled in degenerating testis and waiting for transportation. The copulation probably lasted for a long period, during which the spermatozoa were discharged in batches as spermatophores. These spermatophores were coated with sticky materials secreted from the interstitial areas of testis and the main sperm duct, then transported into ovary.

关 键 词：Sebastes schlegeli germ cell development SPERMATOPHORE copulation period 

分 类 号：S917.4[农业科学—水产科学]