煤工尘肺患者血清中血管内皮生长因子抗体的表达  

The Expression of Vascular Endothelial Growth Factor Antibody in Serum of Coal Workers with Pneumoconiosis

作　　者：程远博[1] 刘素娜[1] 暴磊[1] 张红义[1] 侯建永[1] 郝长付[1] 姚武[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生与职业病学教研室,郑州450000

出　　处：《四川大学学报（医学版）》2014年第5期805-809,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81072278)资助

摘　　要：目的检测煤工尘肺患者及接尘工人血清中血管内皮生长因子抗体（VEGF-Ab）的表达水平,探讨其在尘肺病发病过程中的意义。方法将对象分为4组：以230例煤工尘肺观察对象（已出现尘肺病变但尚未达到壹期尘肺）为观察对象组,以328例煤工尘肺患者为煤工尘肺组,以309例正在接尘的工人为接尘组（尚未出现尘肺病变）,以393例医院健康体检人员为对照组,对所有研究对象进行问卷调查,并采集外周静脉血,分离收集血清,采用酶联免疫吸附试验（ELISA）法检测血清中VEGF-Ab的表达水平。结果煤工尘肺组和接尘组血清中VEGF-Ab表达水平均高于健康对照组和观察对象组（P〈0.05）。随着尘肺病期别的增高,煤工尘肺组患者血清中VEGF-Ab的表达水平虽有增加的趋势,但差异无统计学意义（P〉0.05）。观察对象组中20~岁组和40~岁组VEGF-Ab表达水平均高于60~岁组（P〈0.05）;煤工尘肺组和健康对照组中20~岁组和40~岁组VEGF-Ab表达水平均低于60~岁组（P〈0.05）。煤工尘肺组中采煤组血清VEGF-Ab高于辅助工种组（P〈0.05）,接尘组中掘进组血清VEGF-Ab高于采煤组（P〈0.05）。观察对象组和煤工尘肺组的壹期尘肺组中,工龄〈25年的患者血清中VEGF-Ab表达低于工龄≥25年者（P〈0.05）。结论血清中VEGF-Ab的表达水平可能与煤工尘肺的发生发展过程相关,随着年龄的增大、接尘工龄的增加VEGF-Ab的表达水平升高。Objective To determine the role of serum VEGF-Ab in pneumoconiosis of coal workers.Methods Four groups of participants were recruited for this study,including 230 with early stage（less serious than stage one）changes in relation to pneumoconiosis,328 with confirmed coal worker pneumoconiosis,309 workers exposed to coal dust,and 393 healthy people.All participants completed a questionnaire,and have their peripheral venous blood sample taken.Serum VEGF-Ab was detected by ELISA.Results Compared with healthy controls and those with early stage changes,the participants with pneumoconiosis and those exposed to coal dust had higher levels of serum VEGF-Ab（P 0.05）.The level of serum VEGF-Ab increased with the progression of stages of pneumoconiosis but without statistical significance（P〈0.05）.In those with early stage pneumoconiosis,higher levels of serum VEGF-Ab were found in their 20 yr.-and 40 yr.-compared with those in their 60 yr.-（P〈0.05）.By contrast,in those with confirmed pneumoconiosis and the healthy controls,lower levels of serum VEGF-Ab were found in their 20 yr.-and 40 yr.-compared with those in their 60 yr.-（P〈0.05）.In those with early stage or first-stage pneumoconiosis,longer than 25 years work experience was associated with higher levels of serum VEGFAb（P〈0.05）.In those with confirmed pneumoconiosis,coal mining workers had a higher level of serum VEGFAb than their colleagues involving in assistance tasks（P〈0.05）.In those exposed to coal dust,tunnelling workers had a higher level of serum VEGF-Ab than their coal mining colleagues（P〈0.05）.Conclusion Serum VEGF-Ab is associated with the occurrence and development of coal worker pneumoconiosis.The level of serum VEGF-Ab increases with age and length of exposure to dust.

关 键 词：血管内皮生长因子抗体 煤工尘肺 接尘工人 

分 类 号：R135.2[医药卫生—劳动卫生]