中国鼠疫耶尔森菌染色体结构特征的研究  

Chromosomal structural features of Yersinia pestis isolated from China

作　　者：梁莹[1] 张志凯[1] 赵宏群 蔡虹[1] 夏连续[1] 

机构地区：[1]中国疾病预防控制中心传染病防治所传染病预防控制国家重点实验室,北京102206

出　　处：《中华地方病学杂志》2014年第5期479-484,共6页Chinese Journal of Endemiology

摘　　要：目的 了解中国鼠疫耶尔森菌不同分离株之间的染色体结构差异,探索染色体重排在鼠疫菌中发生的原因及用于菌株遗传特征识别和亲缘关系分析的可能性.方法 以完成全基因组测序的首株鼠疫菌株CO92（美国）为参考株,以从中国分离出的已完成全基因组测序的4株鼠疫菌株（内蒙古91001、云南剑川D182038、云南玉龙D106004、西藏那曲Z176003）为对比株,根据美国国立生物技术信息中心网站（http：//www.ncbi.nlm.nih.gov/genome）上公布的这5株鼠疫菌的染色体序列及染色体上所有编码基因序列间的相似性,将鼠疫菌染色体人为的划分成多个大的DNA节段（染色体板块）,确定这些板块在不同鼠疫菌株间排列方式的差异以及板块之间的断裂区片段的基因组成;根据菌株两两比较所得的重排差异结果,分析菌株间的亲缘关系.结果 除Z176003菌株外,CO92、D182038、D106004、91001菌株可被分成44个相对独立的染色体板块,板块内部基因组成和排列高度稳定,板块之间可以相对移动.与参考株CO92菌株比较,D182038、D106004菌株染色体均存在13处重排,Z176003菌株染色体存在14处重排,91001菌株染色体存在37处板块排列顺序的改变.菌株间两两比较,D106004与Z176003菌株之间仅有8处染色体板块排列方式不同,表明二者的亲缘关系最为接近.CO92菌株染色体上的相邻板块之间存在43个断裂区,有39个断裂区的DNA片段含有插入序列,其中25个插入序列为IS100.结论 鼠疫菌基因组具有一定的流动性,不同菌株间的染色体结构存在较大差异,染色体重排事件发生的原因与其拥有的插入序列密切相关.菌株间染色体的重排差异,能用于菌株遗传特征的识别和亲缘远近关系的分析.Objective To study the differences of chromosomal structure among Yersinia pestis strains isolated from China,and to investigate the reasons of chromosomal rearrangement events occurred in Yersinia pestis as well as the possibility of strain identification and phylogenetic analysis based on the chromosomal rearrangement features.Methods According to the genome sequence data downloaded from web of National Center for Biotechnology Information （http：//www.ncbi.nlm.nih.gov/genome）,alignment of all the coding sequences （CDSs） among five strains（American strain CO92 as reference and other four completely sequenced strains from Inner Mongolia,Jianchuan of Yunnan,Yulong of Yunnan,Naqu of Tibet in China named 91001,D182038,D106004 and Z176003 as comparison strains） was performed,and then the chromosome of Yersinia pestis was divided into several large DNA segments （named chromosomal plate in the text） according to the similarity of CDSs.Plate arrangement patterns in each strain＆#39; s chromosome and gene content of breakpoint regions were determined.Finally,genetic relationships among Yersinia pestis strains were analyzed on the basis of rearrangement diversity from paired-comparison.Results Yersinia pestis chromosomes of strains CO92,D182038,D106004,91001 were composed of 44 relatively independent plates,except strain Z176003.Gene order was very stable within each plate,while it was movable between the plates.Comparing with the reference strain CO92,13 rearrangement events occurred in the chromosomes of both strain D182038 and strain D106004,and 14 rearrangement events involved in Z176003,while 37 rearrangement events occurred in 91001.Paired-comparison data showed that only 8 plates order differences were existed between D106004 and Z176003.Forty-three breakpoint regions were identified on the chromosome of strain CO92,and 39 of them contained insertion sequences,and 25 of them were IS100.Conclusions Yersinia pestis genome represents a high degree of genetic flux,and chromosomal structures of strain

关 键 词：鼠疫耶尔森菌 染色体 基因重排 

分 类 号：R378[医药卫生—病原生物学]