T淋巴细胞亚群、淋巴细胞和C-反应蛋白在急、慢性布鲁杆菌病诊断中的意义  

Diagnosis value of T lymphocyte subgroup, lymphocyte and C-reactive protein in patients with acute and chronic Brucellosis

作　　者：高庆峰[1] 赵冬梅[1] 郭菲[1] 李艳丽[1] 张柠[1] 

机构地区：[1]黑龙江省农垦总局总医院检验科,哈尔滨150088

出　　处：《中华地方病学杂志》2014年第5期565-567,共3页Chinese Journal of Endemiology

摘　　要：目的 探讨T淋巴细胞亚群、淋巴细胞和C-反应蛋白在急、慢性布鲁杆菌病（布病）中的变化,为 辅助诊断布病和急、慢性布病鉴别提供参考.方法 35例急性布病（急性组）、62例慢性布病（慢性组）患者及30例健康体检人群（对照组）血样标本来自于黑龙江省农垦总局总医院检验科.采用细胞流术法和速率散射比浊法分别测定T淋巴细胞亚群（CD3＋、CD19＋、CD3＋/CD4＋、CD3＋/CD8＋）、淋巴细胞和C-反应蛋白.结果 与对照组比较,急、慢性组CD3＋升高（％：74.71±6.79、70.61±8.99比65.00±7.50;F=12.40,P＜ 0.05）,其中急性组高于慢性组（P＜0.05）;CD19＋、CD3＋/CD4＋降低（％：7.79±3.93、9.94±4.53比11.50±3.25;30.56±6.38、35.56±8.22比39.00±4.00;F值分别为18.62、13.03,P均＜0.05）,其中急性组低于慢性组（P均＜0.05）;CD3＋/CD8＋急性组高于对照组和慢性组（％：34.06±835比29.50±7.25、28.72±8.30;F=12.06,P＜0.05）.急、慢性组淋巴细胞高于对照组（％：34.29±10.91、39.12±12.08比30.84±6.45;F=13.78,P＜0.05）,但急性组低于慢性组（P＜0.05）.与对照比较,急、慢性组C-反应蛋白明显升高（mg/L：27.43、15.87比0.60;H=19.42,P＜0.05）,其中急性组高于慢性组（P＜0.05）;C-反应蛋白阳性率组间比较差异有统计学意义（x2=9.94,P＜0.01）,与对照组比较,急、慢性组C-反应蛋白阳性率升高（65.7％、61.3％比3.3％,x2值分别为27.54、23.16,P均＜0.01）.结论 急、慢性布病患者T淋巴细胞亚群、淋巴细胞和C-反应蛋白均发生不同的改变,在辅助诊断布病和急、慢性布病的鉴别诊断与治疗中有一定的参考意义.Objective To investigate the variation of lymphocyte subgroup,lymphocyte and C-reaction protein in patients with acute and chronic Brucellosis,for auxiliary reference in differential diagnosis of acute and chronic Brucellosis.Methods All the blood samples were come from clinical laboratory of Heilongjiang Agricultural Reclamation Bureau General Hospital,which including 35 acute patients（acute group）,62 chronic patients（chronic group） and 30 healthy people（control group）.Flow cytometric method was used to determine the T lymphocyte subgroup（CD3＋,CD19＋,CD3＋/CD4＋,CD3＋/CD8＋） and the lymphocyte.Application of rate scattering turbidimetric method was used for determination of C-reactive protein.Results Percentages of CD3＋ cells in acute and chronic groups were increased compared with that of control group（%：74.71 ± 6.79,70.61 ± 8.99 vs.65.00 ±7.50; F =12.40,P 〈 0.05）,and the acute group was higher than the chronic group（P〈 0.05）.Percentages of CD19＋,CD3＋/CD4＋ cells in acute and chronic groups were decreased compared with that of control group（CD19＋,%：7.79 ± 3.93,9.94 ± 4.53 vs.11.50 ± 3.25; CD3＋/CD4＋,%：30.56 ± 6.38,35.56 ± 8.22 vs.39.00 ± 4.00; F=18.62,13.03,all P〈 0.05）,and the acute group was lower than the chronic group （P〈 0.05）.Percentage of CD3＋/CD8＋ cells in the acute group was higher than those of the chronic and control groups（%：4.06 ± 8.35,29.50 ± 7.25 vs.28.72 ± 8.30; F =12.06,P 〈 0.05）.The lymphocyte counts in acute and chronic groups were higher than that of the control group（%：34.29 ± 10.91,39.12 ± 12.08 vs.30.84 ± 6.45; F=13.78,P 〈 0.05）,but the acute group was lower than the chronic group（P 〈 0.05）.C-reactive protein levels in acute and chronic groups were increased compared with that of the control group（mg/L：27.43,15.87 vs.0.60; H =19.42,P 〈 0.05）,and the acute group was higher than the chronic group（P 〈 0.05）.C-reactive protein positive rates in acute and chronic groups were

关 键 词：布鲁杆菌病 T淋巴细胞亚群 淋巴细胞 C-反应蛋白 

分 类 号：R516.7[医药卫生—内科学]