补肾活血方对良性前列腺增生大鼠前列腺导管系统上皮细胞凋亡的影响  

Bushen Huoxue Fang promotes the apoptosis of epithelial cells in the prostatic ductal system of rats with benign prostatic hyperplasia

作　　者：孙洁[1] 李秋芬[2] 田代志[3] 江少波[1] 邬贤德[1] 裘顺安[1] 任小刚[1] 李玉兵[1] 

机构地区：[1]浙江中医药大学附属第一医院泌尿外科,浙江杭州310006 [2]杭州市中医院肾内科,浙江杭州310000 [3]湖北中医药大学中心实验室,湖北武汉430061

出　　处：《中华男科学杂志》2014年第9期824-829,共6页National Journal of Andrology

基　　金：浙江省医药卫生科技计划(2007B159)

摘　　要：目的:探讨补肾活血方对BPH大鼠前列腺导管系统上皮细胞凋亡的影响及其可能机制。方法:3月龄雄性Wistar大鼠100只,随机分为对照组、去势组、模型组、模型加补肾活血组4组,每组25只。其中模型组、模型加补肾活血组以去势加丙酸睾酮法复制BPH模型。自造模起,模型加补肾活血组给予2.34 g/ml补肾活血方流浸膏灌胃,其余各组予以等体积生理盐水灌胃,共37 d。去势后第38天处死各组大鼠,并留取标本。免疫组化法检测前列腺导管系统转化生长因子-β1(TGF-β1)、α-肌动蛋白分布及阳性细胞数;TUNEL法检测前列腺导管系统上皮细胞凋亡率。结果:与模型组相比,模型加补肾活血组无论前列腺导管近段[(36.42±8.10)%vs(15.28±4.30)%,P<0.01]还是远段[(8.71±2.28)%vs(4.42±2.07)%,P<0.05],TGF-β1阳性细胞百分数均明显升高,但只有近段α-肌动蛋白阳性细胞率高于模型组[(28.14±7.43)%vs(18.28±4.07)%,P<0.01],但仍然低于对照组[(33.57±6.85)%,P<0.05]。与对照组比较,模型组和模型加补肾活血组前列腺腺管远、近段上皮细胞凋亡率均明显下降[远段:17.60±4.86 vs 3.07±1.14(P<0.01)、12.37±2.25(P<0.05);近段:39.42±9.20vs 3.86±1.34(P<0.01)、31.14±5.64(P<0.01)]。与模型组比较,模型加补肾活血组前列腺腺管远、近段上皮细胞凋亡率均明显增高(P<0.01)。结论:补肾活血方可通过上调TGF-β1表达,抑制前列腺导管系统近端的平滑肌数量减少,促进前列腺腺管上皮细胞凋亡,从而有效地抑制了良性前列腺增生。Objective： To investigate the effects of Bushen Huoxue Fang （BSHX） on the apoptosis of epithelial cells in the prostatic ductal system of rats with benign prostatic hyperplasia （BPH） and its possible action mechanism. Methods ： One hundred 3-month-old male Wistar rats were randomly divided into four groups of equal number （ control, castrated, BPH model, and BSHX）. BPH models were made by subcutaneous injection of testosterone following castration ; the rats in the BSHX group were treated intragas- trically with BSHX at 2.34 g/ml after modeling, while those in the other two groups with equal volume of saline, all for 37 days. On the 38th day, all the rats were sacrificed and their prostates harvested for detection of the distribution of TGF-β1 and α-actin and the count of positive cells in the prostatic ductal system by immunohistochemical staining. The apoptosis rate of epithelial cells in the pros- tatic ductal system was determined by TUNEL assay. Results ： The expression of TGF-β1 was significantly increased in the rats of the BSHX group as compared with the BPH models in both the proximal prostatic duct （[ 15.28 ±4.30] % vs [36.42 ± 8.10 ] %, P 〈0.01） and the distal prostatic duct （[4.42 ±2.07]% vs [8.71±2.28]%, P 〈0.05）, while the expression of α-actin in the proximal duct was remarkably higher in the BSHX-treated rats than in the models （ [28.14 ± 7.43 ] % vs [ 18.28±4.07 ] %, P 〈 0.01 ）, but lower than in the control animals （ [ 33.57 ± 6.85 ] %, P 〈 0.05 ）. Compared with the control group, the BPH models and BSHX-treated rats both exhibited markedly decreased apoptosis of epithelial cells in the proximal prostatic duct （[ 39.42 ± 9.20]% vs [3.86 ±1.341%, P〈0. O1, and [31.14 ±5.641%, P 〈0.01） and distal prostatic duct （[17.60±4.861% vs [3.07 ±1.14 ] %, P 〈 0.01, and [ 12.37 ± 2.25 ] %, P 〈 0.05）. The apoptosis rate of epithelial cells in the prostatic ductal system was significantly higher in the BSHX-treated rats than in the BPH m

关 键 词：良性前列腺增生 前列腺导管系统 转化生长因子Β-1 凋亡 中医药 补肾活血方 大鼠 

分 类 号：R285.5[医药卫生—中药学]