钙敏感受体在人骨关节炎软骨中的表达研究  

The Expression of Calcium-Sensing Receptor in Human Osteoarthritis Cartilage

作　　者：刘军[1,2] 曹建刚[1,2] 姚庆斌[3] 李稚君[1,3] 付鑫[2] 马信龙[1,2,4,5] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津市天津医院关节外科 [3]天津医科大学基础医学院 [4]天津医院 [5]天津市中西医结合骨科研究所

出　　处：《天津医药》2014年第9期918-920,I0004,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金重点项目(2013kz063)

摘　　要：目的研究钙敏感受体(CaSR)在人骨性关节炎(OA)软骨中的表达强度,探讨其与OA发病机制的关系。方法收集5例因膝关节OA接受全膝关节置换术患者的股骨远端及胫骨近端标本,HE染色后根据Collins病理学分级标准进行关节软骨退变分级,Collins 0级切片8张为软骨正常组,CollinsⅡ级切片8张为软骨退变组,免疫组织化学染色后比较2组软骨组织内CaSR的表达强度。结果 CaSR在OA软骨中阳性表达,CaSR在软骨正常组的表达强度低于退变软骨组(评分:1.63±0.95 vs 3.52±0.78,t=8.99,P<0.05)。结论 CaSR活化与关节软骨退变有关。Objective To investigate calcium-sensing receptor （CaSR） expression level in osteoarthritis （OA） carti- lage, and the relationship with OA pathogenesis thereof. Methods The degenerated human cartilage of distal femur and proximal tibia came from 5 patients with OA underwent total knee replacement surgery. The tissue specimens were stained by HE. According to the Collins histological grading standards, articular cartilage degeneration was classificated in speci- mens. Eight HE slices of Collins grade 0 were used as normal cartilage group. Eight HE slices of Collins grade 1] were used as degeneration cartilage group. The CaSR expression levels detected by immunohistochemical staining technique were com- pared between two groups. Results Expression of CaSR was detected in osteoarthritis cartilage. The expression intensity of CaSR was significantly lower in normal cartilage group than that of degeneration of cartilage group （score ： 1.63±0.95 vs 3.52± 0.78,t=8.99,P 〈 0.05）. Conclusion The activation of CaSR is associated with the degeneration of articular cartilage.

关 键 词：骨性关节炎 膝关节 软骨细胞 钙敏感受体 免疫组织化学 

分 类 号：R684.3[医药卫生—骨科学]