转化生子因子β_1对培养心脏成纤维细胞α-SMA及Cx43表达的影响  

The effects of TGFβ_1 on the expression ofα-SMA and Cx43 in cultured cardiac fibroblasts

作　　者：王新[1] 王德国[1] 赵春梅[1] 邢文[1] 王安才[1] 朱红军[2] 孙贤林[2] 

机构地区：[1]皖南医学院附属弋矶山医院老年科 [2]安徽省立医院心血管内科

出　　处：《中国临床药理学与治疗学》2014年第7期738-742,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然基金(81200142);省自然科学基金(1208085QH156;1208085MH129);医院人才引进科研基金(YR201001)

摘　　要：目的:本研究拟观察转化生子因子β1(TGFβ1)诱导乳鼠CFs向MFs分化中α-SMA及Cx43表达的影响。方法:酶解-差速贴壁分离法分离培养乳鼠CFs,将10ng/mL的TGFβ1诱导培养第1天细胞;细胞免疫荧光及共聚焦纤维观察α-SMA及Cx43表达;以免疫印迹和RT-PCR分别半定量比较两者α-SMA及Cx43蛋白和mRNA表达。结果:分离培养2d的原代CFs细胞极少表达α-SMA及Cx43蛋白及其mRNA;经TGFβ1诱导24h后α-SMA及Cx43蛋白及其mRNA表达显著增高(P<0.01)。结论:TGFβ1可诱导乳鼠CFs向MFs分化,导致α-SMA及Cx43蛋白及其mRNA表达显著增高。AIM： To observe the effects of transforming growth factor beta-1 （TGFβ1）on the expression of a-smooth muscle actin （α- SMA） and connexin4g （Cx43） in cultured cardi- ac fibroblasts （CFs）. METHODS： CFs were iso- lated from neonatal rat by trypsin dissociation and differentia[ adhension. Primary cultures of CFs for one day were subjected to TGFβ1 （10 ng/ mL） incubation for 24 hours. The expressions of α-SMA and Cx43 were determined by immuno- fluorescence staining. Semiquantitative analysis of mRNA and protein for α-SMA and Cx43 was finished by RT-PCR （reverse transcription poly- merase Chain reaction） and Western blots. RE-SULTS： Little expression of protein and mRNA levels for α-SMA and Cx43 were observed in cul- tured 2 days primary CFs. TGFβ1 stimulation for 24 hours led to significantly increased protein and mRNA expression of α-SMA and Cx43. CONCLUSION： Transient TGFβ1 stimulation causes differentiation of CFs into MFs and up- regulations protein and mRNA expression of SMA and Cx43.

关 键 词：成纤维细胞 肌成纤维细胞 α一平滑肌肌动蛋白 缝隙连接蛋白43 转化生子因子β1 

分 类 号：R965.2[医药卫生—药理学]