罗仙子提取物对单核细胞与血管内皮细胞黏附的影响  

The effects of luoxianzi extractive on the adherence rate of monocyte and vascular endothelial cell

作　　者：刘文彬[1] 褚夫江[1] 吴玉萍[1] 王晖[2] 朱家勇[1] 

机构地区：[1]广东药学院基础学院药用生物活性物质研究所广东省生物活性药物研究重点实验室 [2]广东药学院中药学院

出　　处：《中国临床药理学与治疗学》2014年第7期752-755,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81274061);广东省战略新兴产业核心技术攻关项目

摘　　要：目的:考察罗仙子提取物对单核细胞与血管内皮细胞黏附的影响。方法:制备相对分子质量30 000以下罗仙子提取物;考察罗仙子提取物对血管内皮细胞EA.hy926活力的影响;肿瘤坏死因子(TNF-α)诱导建立单核细胞THP-1和EA.hy926黏附模型,采用虎红染色,考察不同浓度罗仙子提取物对EA.hy926与THP-1黏附性的影响;采用ELISA考察罗仙子提取物对TNF-α诱导的EA.hy926分泌单核细胞趋化蛋白-1(MCP-1)和血管细胞黏附分子-1(VCAM-1)的影响。结果:罗仙子提取物(浓度≤4×102μg/mL时)对EA.hy926未见明显的增殖或抑制作用(P>0.05);罗仙子提取物(浓度≥6.25μg/mL时)可浓度依赖性降低两细胞之间的黏附作用;罗仙子提取物各浓度均能显著地降低TNF-α诱导的MCP-1表达(P<0.01),罗仙子提取物(浓度≥50μg/mL时)可显著地降低TNF-α诱导的VCAM-1的表达(P<0.01)。结论:罗仙子提取物可通过抑制TNF-α诱导的内皮细胞分泌MCP-1和VCAM-1,阻滞单核细胞与血管内皮细胞之间的黏附作用,从而发挥抗动脉粥样硬化作用。AIM： To investigate the effects of luoxianzi extractive on the adherence rate of monocyte and vascular endothelial cell. METH- ODS. The luoxianzi extractive which molecular weight was less than 30 000 was prepared; the influence of luoxianzi extract on vascular endo- thelial cells EA. hy926 s vitality was investiga- ted the THP-1 and EA. hy926 adhesion model induced by tumor necrosis factor （TNF-α） was built using rose bengal staining, investigated the different concentrations of luoxianzi extrac- tive on the adherence effect of THP-1 and EA. hy926; the influence of luoxianzai extract on EA. hy926 secreted MCP-1 and VCAM-1 which induced by TNF-α was investigated by ELISA. RESULTS ： Luoxianzi extract （ concentration ≤4× 102 μg/mL） had no significant inhibition or proliferation effect in EA. hy926（P〉0.05）; lu-oxianzi extract （concentration ≥6.25 /μ/mL） could concentration-dependent decrease the ad- hesion between two cells; all concentrations of luoxianzi extract could significantly reduce the MCP-1 expression induced by TNF-α（P〈 0.01）, luoxianzi extract （concentration ≥50 μg/mL）, significantly reduced the VCAM-1 ex- pression induced by TNF-a. CONCLUSION： Lu- oxianzi extract can reduce TNF-α-induced endo- thelial cells to secrete MCP-1 and VCAM-1, blocking monocyte adherence to vascular endo- thelial cells, and thus play the role of anti-ather- osclerosis.

关 键 词：罗仙子 动脉粥样硬化 黏附性 血管内皮细胞 

分 类 号：R965.2[医药卫生—药理学]