内吗啡肽-1对麻醉大鼠左心室功能的影响  

Effect of endomorphin-1on the left ventricular function in anesthetized rats

作　　者：程向阳[1] 宗巧凤 胡杰[2] 高琴[2] 李正红[2] 

机构地区：[1]蚌埠医学院第一附属医院麻醉科 [2]蚌埠医学院生理学教研室

出　　处：《中国临床药理学与治疗学》2014年第8期872-876,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省高校省级自然科学研究重点项目(KJ2011A202);安徽省高校省级自然科学研究项目(KJ2012Z246)

摘　　要：目的:观察内吗啡肽-1(endomorphin-1,EM-1)静脉注射和侧脑室注射对麻醉大鼠左心室功能的影响,并初步探讨其可能的作用机理。方法:Wistar大鼠麻醉后,静脉注射或侧脑室注射EM-1,经右颈总动脉左心室插管测定左心室功能,并测定血清和心肌组织NO和T-NOS含量。结果:静脉注射、侧脑室注射EM-1均剂量依赖性地降低麻醉大鼠左心室功能,预先给予阿片受体阻断剂纳洛酮或特异性μ受体阻断剂cyprodime可阻断EM-1引起的心功能降低反应;预先给予胆碱能M受体阻断剂阿托品可减弱EM-1引起的心功能降低反应。静脉注射EM-1血清中NO和T-NOS比对照组有所增加,而心肌组织的NO和T-NOS无明显变化;侧脑室注射EM-1的血清和心肌组织的NO和T-NOS均无明显变化。结论:静脉注射、侧脑室注射EM-1可引起麻醉大鼠在体左心室功能下降,此效应由阿片受体介导,可能有胆碱能M受体参加。AIM： To observe the effects of in- travenous and intracerebroventrieular injection of endomorphin-l（EM-1） on the left ventricular function in anesthetized rats and to assess its mechanism. METHODS： The left ventricular function was observed by inserting a catheter from right carotid artery into left ventricle after iv or civ injection of EM-1 in anesthetized rats. The effects of iv or civ of various blockers, nal- oxone, cyprodime and atropine, on the left ven- tricular function caused by EM-1 were observed. The level of NO and T-NOS in serum and myo- cardial were measured. RESULTS： EM-1 was shown to decrease left ventricular function dose- dependently. The decrease in left ventricular function was blocked by naloxone and cyprodime. Pretreatment with iv or civ atropineattenuated the decrease response. Intravenous injection of EM-1 produce NO content and NOS activity in serum were much higher than those in control group （P〈0.05）. While NO content and NOS activity in myocardium were no more than those in control group. Intracerebroventric- ular injection of EM-1 produced NO content and NOS activity in serum and myocardium were no more than those in control group. CONCLU- SION： iv or civ administration of EM-1 produces depressor on the left ventricular function in an- esthetized rats, which is mediated by opioid re- ceptor, associated with the Mcholinoceptor.

关 键 词：内吗啡肽-1 阿片受体 左心室功能 一氧化氮 

分 类 号：R965[医药卫生—药理学] R331[医药卫生—药学]