河北省苹果园根际土壤中疑似致病镰孢菌种类  

Suspected pathogenic Fusarium spp. isolated from apple orchard soils in Hebei Province

作　　者：邹庆甲[1,2] 王树桐[1] 梁魁景[3] 王亚南[1] 胡同乐[1] 韩之琪 曹克强[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]邢台市园林局,河北邢台054001 [3]衡水学院生命科学学院,河北衡水053000

出　　处：《菌物学报》2014年第5期976-983,共8页Mycosystema

基　　金：公益性行业(农业)科研专项经费项目(No.200903004-42);国家苹果产业技术体系项目(No.nycytx-08-04-01)

摘　　要：为了解引起河北省苹果再植病害的病原菌,在河北省10个地区苹果园中采集土壤样品,在实验室进行病原菌的诱集分离培养,根据形态和分子特征对主要病原菌进行种类鉴定。结果表明,在分离得到的293株真菌中,有116株镰孢菌,为分离频率最高的真菌。在形态学鉴定的基础上,对供试镰孢菌进行了分子鉴定。在基于核糖体基因内转录间隔区(rDNA‐ITS)序列与翻译延长因子1α(EF‐1α)序列片段构建的系统发育树中,代表菌株分别与GenBank登记的所属菌株聚于同一群。研究结果明确了河北省苹果再植病害的疑似致病镰孢菌,包括:尖孢镰孢Fusarium oxysporum、木贼镰孢F.equiseti、锐顶镰孢F.acuminatum、层出镰孢F.proliferatum和茄腐镰孢F.solani。The pathogenic fungi of apple replant diseases were baited from crabapple orchard soil samples collected from10 regions in Hebei Province. Among the 293 isolates, 116 isolates were Fusarium species, which were the predominant group in the soil fungi. The phylogenetic trees inferred from internal transcribed spacers regions （rDNA-ITS） and partia sequence of translation elongation factor-la gene （EF-la） showed that the representative isolates were all clustered with the Fusarium species blasted in GenBank. The suspected pathogenic Fusarium species in apple orchard soil in Hebe Province included Fusarium oxysporum, F. equiseti, F. acuminatum, F. proliferatum and F. solani

关 键 词：苹果再植病害 镰孢菌 鉴定 INTERNAL transcribed spacers(ITS) EF一1α 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]