利用ISSR标记分析红脂大小蠹微生境中球孢白僵菌遗传多样性  

Genetic diversity of Beauveria bassiana in the microhabitat of Den-droctonus valens using ISSR molecular makers

作　　者：陈雪娇[1] 李云飞[1] 孙娟娟[1] 宗凯[1] 余晓峰[1] 郑海松[1] 姚剑[1] 

机构地区：[1]安徽出入境检验检疫局技术中心,安徽合肥230022

出　　处：《菌物学报》2014年第5期1015-1024,共10页Mycosystema

基　　金：安徽省自然科学基金(No.70411029);国家质检总局科技计划项目(No.2009IK268)

摘　　要：针对红脂大小蠹危害程度不同的3个地区的球孢白僵菌种群,利用ISSR(inter‐simple sequence repeat)分子标记分析了各个种群的遗传多样性。从19条引物中筛选出10条多态性高、稳定性好的ISSR引物用于扩增分析。68株球孢白僵菌的Nei’s基因多样性(h)为0.2973,Shannon指数(Is)为0.4488。旬邑、宜君、古交3地白僵菌种群间的基因分化系数(GST)为0.0525,基因流(Nm)为9.0255;而来源于土壤、红脂大小蠹、蛀屑和树皮的白僵菌种群间的基因分化系数为0.1449,基因流为2.9508。各球孢白僵菌种群表现出不同的多样性水平,旬邑种群和红脂大小蠹虫种群的遗传多样性相对较高;地理分布种群间的差异不如分离基质种群间的差异明显,地理分布种群间存在明显的基因交流,而分离基质种群间的基因流较低,遗传分化明显。ISSR molecular marker was used to analyze the genetic diversity of Beauveria bassiana populations from three areas with different damage extent caused by the red turpentine beetle Dendroctonus valens. Ten out of 19 ISSR primers were selected for PCR amplification with high yields of polymorphism and stability. The results demonstrated that the genetic diversity of Beauveria bassiana varied among populations. Diversity level of Xunyi population and beetle population was relatively higher than the others. The genetic difference among geographic origins was less than those of matrix population. Considerable gene flow was detected in geographic populations. However, the indices of gene flow amongmatrix populations were relatively lower but with apparent genetic differentiation among populations.

关 键 词：球孢白僵菌 红脂大小蠹 锚定简单序列重复 遗传多样性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]