一株无花果内生真菌及其次生代谢产物  

An endophytic fungus in Ficus carica and its secondary metabolites

作　　者：王丽薇[1] 王国平 唐婷[1] 邢旺兴[1] 郑威[1] 王靖[1] 章初龙[3] 

机构地区：[1]杭州师范大学医学院药学系,浙江杭州310036 [2]浙江大化生物医药研发中心,浙江杭州311616 [3]浙江大学生物技术研究所,浙江杭州310058

出　　处：《菌物学报》2014年第5期1084-1093,共10页Mycosystema

基　　金：浙江省自然科学基金(No.LQ13H280005);杭州师范大学实验室开放项目(No.2013040)

摘　　要：从无花果Ficus carica叶中分离获得1株具有抗菌活性的内生真菌ZJWCF255,通过形态学和ITS rDNA序列分析将菌种鉴定为炭角菌属Xylaria sp.,其发酵液经活性跟踪分离,采用硅胶柱层析及HPLC等方法获得4个化合物。根据波谱数据,化合物1–4分别鉴定为cytochalasin C、D、Q、R。Cytochalasin Q(3)对11种植物病原真菌均具有较强的抑制活性,对蔓枯病菌的抑制率最强,EC50值为0.04μg/mL。该化合物对3种肿瘤细胞SMMC‐772、MCF‐7、MGC80‐3具有较强体外抑制活性,其IC50值分别为(17.24±2.55)、(7.75±1.37)、(10.30±1.34)μg/mL。首次报道了cytochalasin Q(3)的抗菌活性及对3种肿瘤细胞的体外抑制活性,植物内生真菌是获得抗菌与抗肿瘤活性先导化合物的重要来源。An endophytic fungus ZJWCF255 with antifungal activity was isolated from the leaf of Ficus carica, and identified as Xy1aria sp. on the basis of morphology and ITS rDNA sequence data. Four compounds were obtained fromthe fermentation broth of the endophyte by silica gel column chromatography and high performance liquid chromatography （HPLC） through bioassay-guided isolation. Their structures were elucidated as cytochalasin C （1）, D （2）, Q （3）, and R （4） through spectral analysis. The primary bioassay results showed that compound 3 had inhibitory activity to 11 plant pathogenic fungi, especially to Didymella bryoniae, with ECso value of O.04μg/mL. Compound 3 also showed strong antitumor activity on tumor cell lines SMMC-772, MCF-7, MGC80-3 in vitro with ICs0 values of （17.24±2.55）, （7.75±1.37）, （10.30±l.34）μg/mL respectively. This is the first report of antifungal effect and antitumor activity of cytochalasin Q.

关 键 词：植物内生菌 炭角菌 细胞松弛素 抗菌活性 抗肿瘤活性 

分 类 号：S663.3[农业科学—果树学]