超小超顺磁性氧化铁增强MRA联合炎症因子检测兔动脉粥样硬化易损斑块  

Ultrasmall superparamagnetic iron oxide-enhanced magnetic resonance angiography combined with inflammatory factors for detecting atherosclerotic plaques in rabbits

作　　者：李公信[1] 王露朝 刘芃[1] 温志波[1] 黄凡衡[1] 陈立桁 赵鑫[1] 林霖[1] 周怡军[1] 

机构地区：[1]南方医科大学珠江医院心内科,广东广州510282

出　　处：《南方医科大学学报》2014年第9期1324-1328,共5页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2011010004025)

摘　　要：目的探讨超小超顺磁性氧化铁(USPIO)增强MRA联合IL-6、IL-I0检测兔动脉粥样硬化(AS)易损斑块的可行性及价值。方法健康雄性新西兰大白兔24只,分为A、B、C 3组。A组采用球囊导管损伤主动脉内膜结合高脂饲养建立AS模型,B组采用高脂饲养,空白C组不做干预。12周于耳缘静脉采血5 ml比较各组间血脂及IL-6、IL-10的变化情况。行MRA平扫、USPIO增强扫描,与病理检查结果行对照研究。结果 A、B两组比较血脂水平无统计学意义(P>0.05),IL-6水平(167±21.3)pg/ml vs(116±14.3)pg/ml P<0.05,IL-10无差异(P>0.05)。A vs C、B vs C(TC、TG、LDL)P<0.01,HDL无差别。IL-6、IL-10有显著性差异(P<0.01)。MRA连续扫描中,管壁信噪比(SNR)在不同组间存在差异(P<0.05)。结论 USPIO通过标记巨噬细胞引起T2WI信号下降,结合SNR峰值变化可以预测兔动脉粥样硬化血管整体的炎症程度。USPIO增强MRA联合IL-6、IL-IO检测兔AS易损斑块具有可行性。Objective To investigate the feasibility of Ultrasmall superparamagnetic iron oxide-enhanced magnetic resonance angiography （USPIO-MRA） combined with interleukin-6 （IL-6） and IL-10 detection for detecting atherosclerotic plaques in rabbits. Methods Twnety-four normal male rabbits were randomly assigned （n=8） into group A with atherosclerosis induced by damaging the aortic tunica intima with Foley＇s tube in combination with a high fat diet, group B with a high fat diet, and group C without any intervention. At week 12, plain and USPIO-MRA was performed in all the 24 rabbits and the results were compared with pathological examinations; blood samples were collected from the ear vein to examine blood lipids and levels of IL-6 and IL-10. Results The rabbits in groups A and B showed significantly different IL-6 levels （167±21.3 vs 116±14.3 pg/ml, P〈0.05） but comparable blood lipids and IL-10 levels （P〉0.05）. The levels of IL-6, IL-10, TC, TG, and LDL, but not HDL, differed significantly between groups A and C and between groups B and C （P〈0.01）. Continuous MRA scan showed significantly different signal-to-noise ratios （SNR） between the 3 groups. Conclusion USPIO-MRA combined with IL-6 and IL-10 detection is feasible in detecting atherosclerotic plaques in rabbits.

关 键 词：动脉粥样硬化 超小超顺磁性氧化铁 白细胞介素6 白细胞介素10 

分 类 号：R543.5[医药卫生—心血管疾病]