细胞因子CCR9/CCL25对鼻咽癌细胞CNE-2增殖的影响  

Effect of Chemokines CCR9/CCL25 on the Proliferation of NPC Cell CNE-2

作　　者：叶林峰[1] 张利平[1] 肖伟[1] 吴婷婷[1] 

机构地区：[1]武汉大学中南医院耳鼻咽喉-头颈外科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2014年第5期689-691,共3页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金资助项目(编号:2009CDB271)

摘　　要：目的:探讨CCR9/CCL25通路对鼻咽癌CNE-2细胞增殖的影响。方法:培养CNE-2细胞,在培养体系中加入不同浓度的CCL25(0,10,25,50和100ng/ml),用MTT法检测各组细胞的增殖率,确定促CNE-2细胞增殖的最佳CCL25浓度。然后在培养体系中同时加入不同浓度的anti-CCR9(0,0.25,0.5,1,2μg/ml),观察此时CNE-2细胞的增殖情况。结果:不同浓度的CCL25均能促进CNE-2细胞的增殖(P<0.05)。而且其促细胞增殖作用呈现浓度依赖性,50ng/ml时达到最高水平。Anti-CCR9能阻断CCL25的促CNE-2增殖作用(P<0.05),而且也呈浓度依赖性。结论:CCL25具有促进CNE-2细胞增殖的作用,并呈现浓度依赖性。Anti-CCR9能阻断CCL25的促CNE-2增殖作用,并呈浓度依赖性。Objective：To investigate the effect of CCL25 on the proliferation of NPC cell line CNE-2.Methods：For determining the optimized concentration of CCL25,we detected the proliferation rate in each subgroups of cultured CNE-2cells treated with CCL25.Then the proliferation status of CNE-2cells were observed after anti-CCR9 of different concentrations（0,0.25,0.5,1,and 2μg/ml）was simultaneously added into the culture systems.Results：All these studied concentrations of CCL25 could promote the CNE-2proliferations（P〈0.05）.This promoting effect presented a feature of concentration-dependence and reached to the peak at 50ng/ml.Anti-CCR9 could block this promoting effect（P 0.05）also in a concentration-dependent manner.Conclusion：The CCL25 could facilitate the proliferation of CNE-2cells in a concentration-dependent manner.Anti-CCR9 could block the promoting effect of CCL25 on CNE-2cells and is also concentration dependent.

关 键 词：鼻咽癌 CNE-2细胞 细胞因子 CCR9/CCL25 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]