5-羟色胺转运体敲除小鼠在高架十字迷宫中下丘脑CRF mRNA表达的变化  

Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze

作　　者：文玲[1] 邓永兵[1,2] 朱小云[1] 蒋雪[1] 

机构地区：[1]重庆医科大学生物化学与分子生物学教研室,重庆400016 [2]重庆市急救医疗中心神经外科,重庆400014

出　　处：《第三军医大学学报》2014年第18期1876-1880,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金(81001017);重庆市教育委员会科技基金(渝教科文2010-5);重庆市卫生局科技基金(渝教科文2012-2-254)~~

摘　　要：目的研究5-羟色胺转运体(serotonin transporter,SERT)敲除小鼠在高架十字迷宫(elevated plus maze,EPM)中下丘脑促肾上腺皮质激素释放因子(corticotrophin-releasing factor,CRF)mRNA表达的变化。方法将小鼠按基因型(SERT+/+、SERT+/-、SERT-/-)分为对照组和EPM组,每组10只,EPM组利用高架十字迷宫作为应激原刺激小鼠。测定各组小鼠下丘脑以及室旁核部位CRF mRNA的表达。结果在基础条件下(对照组),SERT敲除小鼠下丘脑及室旁核的CRF mRNA表达降低(P<0.05);EPM刺激下(EPM组),SERT+/-和SERT-/-小鼠在下丘脑及室旁核的CRF mRNA表达显著高于SERT+/+(P<0.01);构建的CRF mRNA探针反义链在下丘脑PVN部位检测到高密度的CRF mRNA,而正义链却不能探测到CRF mRNA;EPM组hnRNA SERT-/-小鼠表达较对照组明显增高(P<0.01)。结论应激能明显促进下丘脑CRF mRNA的表达。SERT敲除小鼠的异常应激与CRF mRNA的表达升高有关。Objective To determine the expression alteration of corticotrophin-releasing factor（CRF）mRNA in the hypothalamus of serotonin transporter（ SERT）-knockout mice exposed to elevated plus maze（EPM）. Methods Mice were divided into 2 groups,a control group and an EPM group,according their genotypes（SERT＋ / ＋,SERT＋ /-,and SERT- /-）,with 10 mice in each group. EPM was used as a stressor to stimulate the mice. Then anxious like behavior was observed, and the CRF mRNA expression in the hypothalamus and nucleus paraventricularis（ PVN） were measured. Results The non-stress expression of CRF mRNA in the hypothalamus and PVN were lower in both SERT＋ /-and SERT- /-mice than in SERT＋ / ＋mice（P 0. 05）. Exposure to the EPM significantly increased CFR mRNA in the hypothalamus and PVN of SERT＋ /-and SERT- /-mice（ P 0. 05）,higher than that of the SERT＋ / ＋mice. CRF mRNA antisense probes in the PVN detected high density of CRF mRNA,but CRF sense mRNA did not. With exposure to the EPM,the expression of hnRNA in SERT- /-mice was significantly higher than that in the control group（P 0. 01）. Conclusion Stress from exposure to EPM significantly increases CRF mRNA expression in the PVN and hypothalamus of SERT＋ /-and SERT- /-mice. The abnormal stress reaction of SERT-knockout mice is associated with the increased CRF mRNA expression.

关 键 词：5-羟色胺转运体 高架十字迷宫 下丘脑 室旁核 肾上腺皮质激素释放激素 

分 类 号：R338.27[医药卫生—人体生理学] R363.27[医药卫生—基础医学]