不同培养基及激素处理下柽柳丛生芽总酚、三萜及黄酮物质的积累  

Accumulation of total phenolics,triterpenoids and flavonoids in tufted buds of Tamarix chinensis under different medium and hormone treatment

作　　者：王思瑶[1] 李明阳[1] 邵占媛 尹静[1] 

机构地区：[1]东北林业大学生命科学学院林木遗传育种国家重点实验室

出　　处：《北京林业大学学报》2014年第5期74-81,共8页Journal of Beijing Forestry University

基　　金：东北林业大学国家级大学生创新训练项目(201310225025);国家基础科学人才培养基金项目(J1210053);哈尔滨市青年创新基金项目(2012RFQXN007)

摘　　要：以内蒙柽柳嫩枝为外植体,分别进行8种激素组合（2水平NAA和4个水平6-BA）及5种类型培养基（MS、12 MS、NT、B5和WPM）处理,研究柽柳组培苗生长和次生产物含量积累变化。结果表明：不同激素组合及不同培养基类型均对柽柳组培苗生长和次生产物积累有显著影响,其中柽柳丛生芽最佳生长培养基为MS附加激素6-BA1.0 mgL＋NAA 0.05 mgL。各处理下柽柳丛生苗组织中总酚含量均较高（136.84-353.58 mgg）,其次为总三萜（17.65-63.13 mgg）,总黄酮含量相对较低（11.10-18.92 mgg）。在附加激素为6-BA 1.0 mgL＋NAA 0.05 mgL的不同类型培养基条件下,柽柳组培苗一个生长周期（30 d）内的总酚产量以12 MS最高,可达60.76 mg;总三萜产量以MS培养基最高,可达9.13 mg;总黄酮产量以12 MS和MS培养基较高,分别为3.25和2.50 mg。In this study, the shoots of Tamarix chinensis were treated with eight kinds of hormone combinations （two level NAA and four level 6-BA） and five types medium （ MS, 1/2 MS, NT, B5 and WPM） and the growth and contents of secondary metabolite products were investigated in tamarix plantlet. The results showed that different hormone combinations and different medium had significant effects on the growth and accumulation of secondary products of T. chinensis seedlings. MS supplemented with 6-BA （ 1.0 mg/L） and NAA （0.05 mg/L） resulted in the optimal growth of T. chinensis. Under different treatments, the highest secondary product was phenol （ 136.84 - 353.58 mg/g） , followed by triterpenoids （ 17.65 - 63.13 mg/g） and total flavonoids content （11.10 - 18.92 mg/g）. When hormones 6-BA （1.0 mg/L） and NAA （0.05 mg/L） were added into different type medium, total phenolic yield of the 1/2 MS was the highest, while the total triterpenoids production in MS medium was the highest （up to 9.13 rag）. Total flavonoids yield of 1/2 MS and MS medium was 3.25 and 2.50 mg, respectively. This study lays a foundation for the large-scale production of T. chinensis medicinal ingredients through the process of biological fermentation.

关 键 词：柽柳 组织培养 丛生芽 次生代谢 

分 类 号：S718.43[农业科学—林学]