盐碱胁迫对哈茨木霉T28的生长及营养代谢的影响  

Effects of salt-alkali stress on growth and nutritional metabolism of Trichoderma harzianum T28

作　　者：尹大川[1] 邓勋[2] 郑宇[1] 宋瑞清[1] 

机构地区：[1]东北林业大学林学院 [2]黑龙江省林业科学院森林保护研究所

出　　处：《北京林业大学学报》2014年第5期109-116,共8页Journal of Beijing Forestry University

基　　金："十二五"国家科技支撑计划项目(2012BAD19B0801);国家自然科学基金项目(31170597;31200484);黑龙江省森工总局项目(SGZJY2010014);"948"国家林业局引进项目(2009-4-39)

摘　　要：采用菌物学、生物化学等方法,对从以色列引进的哈茨木霉T28进行盐碱胁迫研究,从菌株生长和营养代谢的角度研究了该菌株对盐碱胁迫的响应。结果表明：在NaCl、Na2SO4和NaHCO3胁迫条件下,菌株生长和营养代谢与盐碱浓度呈明显的相关性,在cNaCl≤0.4 mol/L和cNa2SO4≤0.2 mol/L胁迫的56 h内,T28均可长满平板（Φ=90mm）,生物量为0.17-0.38 g/150 mL和0.51-0.52 g/150 mL,碳利用率为16%-43%和8.4%-8.7%,磷利用率为3.3%-4.7%和4.3%-5.7%。菌株生长和对碳、磷相对利用率受抑制程度均较低;在NaHCO3胁迫下,菌株生长和营养代谢均受到较强的抑制。上述结果表明,T28表现为较好的耐盐性和碱胁迫不耐受。The response of Trichoderma harzianum strain T28 （introduced from Israel） to salt-alkali stress was researched using mycology and biochemistry. The research investigated strain growth and nutritional metabolism. The results showed that under the stresses of NaC1, Na2SO4 and NaI-ICO3, growth and nutritional metabolism were significantly correlated to salt-alkali concentrations. Within 56 hours, the diameters of T28 colonies for both cNaC1≤0.4 mol/L and cNa2SO4≤0.2 mol/L had reached 90 mm; the biomass readings were 0. 17-0.38 g/150 mL and 0.51-0.52 g/150 mL; the utilization rates of carbon were 16%-43% and 8.4%-8.7%; and the utilization rates of phosphorus were 3.3%-4.7% and g. 3% -5.7% respectively. The inhibition on strain growth and utilization rates of carbon and phosphorus were lower. Under NaHCO3 stress, the inhibition on strain growth and utilization rates of carbon and phosphorus were higher. It is concluded that strain T28 shows a high tolerance to salt and intolerance to alkaline stress.

关 键 词：哈茨木霉T28 盐碱胁迫 生长 营养代谢 

分 类 号：Q935[生物学—微生物学]