光动力治疗前后宫颈尖锐湿疣皮损Toll样受体7和9及α干扰素和干扰素调节因子7的表达  

Effects of photodynamic therapy on the expression of TLR7, TLR9, IFN-α and IRF-7 in cervical condyloma acuminata

作　　者：李蕾[1] 邹先彪[1] 张云杰[1] 陈红霞[1] 郭小娟[1] 曲莉[1] 林卉[1] 

机构地区：[1]解放军总医院第一附属医院皮肤科,北京100048

出　　处：《实用皮肤病学杂志》2014年第5期330-333,共4页Journal of Practical Dermatology

基　　金：解放军总医院科技创新苗圃基金(11KMM31);国家自然科学基金青年基金资助项目(61308117);解放军总医院百项优势基金资助项目

摘　　要：目的观察光动力治疗前后宫颈尖锐湿疣皮损Toll样受体(TLR)7和TLR9及α干扰素(IFN-α)和干扰素调节因子(IRF)-7表达量的变化。方法采用实时定量荧光聚合酶链反应(PCR),检测宫颈尖锐湿疣皮损在光动力疗法治疗前后TLR7、TLR9、IFN-α及IRF-7的表达,并进行对比分析。结果 15例患者宫颈部位皮损光动力治疗前可检测到TLR7、TLR9、IFN-α及IRF-7的表达,而且表达比正常对照组高。经过光动力治疗后,局部皮损TLR7、TLR9、IFN-α及IRF-7的表达量下降,前后对比差异具有统计学意义。结论光动力治疗后宫颈尖锐湿疣皮损TLR7、TLR9、IFN-α及IRF-7等免疫受体及细胞因子表达下降,提示光动力治疗尖锐湿疣前后局部免疫状态发生变化。Objective To study the expression changes of TLR7, TLR9, IFN-αand IRF-7 in the lesions of cervical condyloma acuminata（CA） before and after photodynamic therapy. Methods The expression of TLR7, TLR9, IFN-αand IRF-7 in cervix CA lesions were detected by real-time lfuorescent quantitative PCR method and analyzed before and after photodynamic therapy. Results The expression of TLR7, TLR9, IFN-αand IRF-7 in the cervical lesions of 15 patients were detected before and after photodynamic therapy. For the untreated lesions, the expression level of IRF-7, TLR7, TLR9 and IFN-α was higher than the normal control group; however, after photodynamic therapy, the expression level of IRF-7, TLR7, TLR9 and IFN-αdecreased signiifcantly. Conclusion For the treatment of cervical condyloma acuminata, photodynamic therapy may cause the expression of immune receptors and cytokines decreased, such as TLR7, TLR9, IFN-αand IRF-7, it suggest that the mechanism of photodynamic therapy curing cervical condyloma acuminata may involve immune mechanism.

关 键 词：尖锐湿疣 光动力疗法 TOLL样受体 α干扰素 干扰素调节因子7 

分 类 号：R752.53[医药卫生—皮肤病学与性病学] R454.2[医药卫生—临床医学]