酶催化动力学光度法测定铜(Ⅱ)  

Enzymatic Spectrophotometric Determination of Copper(Ⅱ)

作　　者：柳畅先[1] 朱苗苗[1] 王艺[1] 

机构地区：[1]中南民族大学化学与材料科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2014年第3期24-26,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学自然科学基金资助项目(XTZ09005)

摘　　要：基于脲酶(UASE)催化尿素水解的反应中Cu2+对于酶的抑制作用原理,通过测定产物吸光度的变化率得出了其酶促反应速度,对应于Cu2+浓度而制得标准曲线,检出限为0.05μmol/L.讨论了pH值和温度对测定的影响,结果表明:该法快速灵敏.A spectroscopic method was developed to determine copper （Ⅱ） content based on the inhibition of copper （Ⅱ） for the hydrolysis of urea catalyzed by urease （ UASE） .The rate of the absorbance change of the product was measured for the calculation of the rate of the enzymatic reaction and a standard curve of copper （Ⅱ） was prepared .The detection limit could reach 0.05μmol/L, and the effects of the pH value and temperature were also discussed .The results indicated that the method was rapid and sensitive .

关 键 词：铜(Ⅱ) 测定 动力学光度法 Cu(Ⅱ) 

分 类 号：O657.3[理学—分析化学] X832[理学—化学]