MMP3基因启动子rs102715950(5A/6A)单核苷酸多态性与河南人群食管鳞癌易感性的关系  

Association between SNP rs102715950(5A/6A) of MMP3 Gene and Susceptibility to Esophageal Squamous Cell Carcinoma in Different Groups of People in Henan

作　　者：李劲[1] 李可可[1] 姚品芳[2] 陈静波[2] 陈真[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]湖北省肿瘤医院,武汉430079

出　　处：《中南民族大学学报（自然科学版）》2014年第3期37-40,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：武汉市科技计划项目攻关计划资助项目(SZY13004)

摘　　要：为研究MMP3基因5'端转录调控区rs102715950(5A/6A)单核苷酸多态性与河南人群食管癌易感性的关系,通过蛋白酶K法提取基因组DNA,Nested PCR扩增目的基因片段,用Tth111I进行酶切,PAGE电泳检测酶切结果,对河南散发、正常、高发区3种人群进行了MMP3基因SNP rs102715950(5A/6A)基因分型,用SPSS软件作了Logistic回归统计分析.结果表明:在MMP3基因rs102715950(5A/6A)单核苷酸多态性上,河南食管癌散发人群、河南正常人群、湖北的河南移民人群3类人群相互之间并无显著性差异(p>0.01),说明MMP3基因rs102715950(5A/6A)单核苷酸多态性与河南人群的食管鳞癌易感性无显著相关性.To explore the association between SNP rs 102715950（5A/6A） in 5′-end regulation region of MMP3 gene and susceptibility to esophageal squamous cell carcinoma （ ESCC ） in different groups of people in Henan , Genomic DNA was extracted from peripheral blood with the proteinase K method .The genotyping was carried out by PCR-RFLP and analyzed comparatively.Then the results of genotyping were analyzed by SPSS 19.0.The results showed that there was no statistical differences in the genotyping of MMP3 SNP rs102715950（5A/6A） of among 3 groups of people ,including sporadic cases of esophageal cancer , migrants of the high incidence area and healthy controls in Henan .So there was no obvious correlations between the MMP3 SNP rs102715950（5A/6A） with the susceptibility of esophageal squamous cell carcinoma among the people in Henan .

关 键 词：MMP3基因SNP 河南不同人群 基因分型 PCR-RFLP logitic回归分析 

分 类 号：Q354[生物学—遗传学]