人体牙髓干细胞的分离与鉴定  

Isolation and characterization of Human Dental Pulp Stem Cells

作　　者：张男 王伟[1,2] 陈保兴[1,3] 陈超[1,2] 贾昕[1,4] 丁宇欣 左鑫[1,4] 邓琴南 张彬[1,2] 韩发彬[1,2] 

机构地区：[1]泰山医学院聊城临床学院 [2]聊城市人民医院干细胞与再生医学重点实验室,聊城252000 [3]聊城市人民医院口腔颌面实验室,聊城252000 [4]聊城市人民医院脑科医院实验室,聊城252000 [5]Dr.Samuel QN.Deng Dental Clinic

出　　处：《中华细胞与干细胞杂志（电子版）》2014年第3期32-35,共4页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：山东省博士后创新基金(201303025)

摘　　要：目的探讨牙髓干细胞(DPSC)对牙周病,外伤及肿瘤等造成下颌骨缺损、口腔软组织与神经损伤的修复治疗作用。方法本研究利用组织块培养法分离出人体DPSC,用流式细胞仪进行了鉴定,并进行DPSC成骨、成脂、成神经的分化研究。结果分离出3株DPSC,流式细胞分析表明DPSC表达CD73和CD90标志物,但不表达生血干细胞标志物CD34。用茜素红染色表明DPSC能分化成骨细胞,油红O染色表明DPSC能分化成脂肪细胞,免疫免疫荧光染色表明DPSC分化的细胞表达神经细胞特异标志物TUJ1。结论组织块培养能够高效快速分离表达CD73和CD90的DPSC,在体外诱导条件下DPSC能分化为成骨细胞、脂肪细胞和神经细胞,此研究为DPSC在治疗和修复骨组织缺损和神经损伤中的临床应用提供了实验依据。Objective To explore the repairing ability of dental pulp stem cell （DPSC） in mandibular bone defects, soft tissue and neural injuries from oral tumors, trauma and periodontal diseases. Methods DPSC were isolated from dental pulp tissues and induced to differentiate into osteogenic cells, adipose cells or neural cells which were identified by alizarin red staining, oil red O staining, or immunofluorescence respectively. CD antigen of DPSC was detected by flow cytometry. Results Human DPSCs were isolated from the dental pulp tissues. Flow cytometry analysis indicated that DPSCs from three dental pulps expressed CD73 and CD90, but not CD34. Alizarin red, oil red O, or immunofluorescence staining indicated that DPSC were able to differentiate into osteogenic cells, adipose cells, and neural cells respectively. Conclusion Dental pulp tissue is rich in DPSCs. DPSCs have the clinical potential to be used as stem cell sources for repairing bone defects and neural injuries.

关 键 词：牙髓 干细胞 流式细胞术 免疫荧光技术 细胞分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]