一种棉花细胞质雄性不育系线粒体RNA的提取方法  

A Method for Mitochondrial RNA Isolation of Cytoplasmic Male Sterility in Cotton

作　　者：巩养仓[1] 张兴平[1] 邢朝柱[2] 王洪[1] 吴建勇[2] 郭立平[2] 彭凡嘉[1] 

机构地区：[1]湖南省棉花科学研究所,湖南常德415101 [2]中国农业科学院棉花研究所,河南安阳455000

出　　处：《安徽农业科学》2014年第29期10097-10098,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]获得高质量的棉花细胞质雄性不育系线粒体RNA。[方法]以哈克尼西棉细胞质雄性不育系黄化苗为研究对象,对棉花线粒体RNA的提取方法进行探讨。[结果]采取先分离出棉花线粒体再提取RNA的方法,最终获得了质量较好的线粒体RNA(mtRNA)。[结论]成功获得了质量较高的mtRNA。The aim was to obtain high quality mitochondrial RNA isolation of cytoplasmic male sterility in cotton. [Method]Taking CMS line of G. harknessii as material,a method of mitochondrial RNA isolation was constructed. [Result]The protocol was that cotton mitochondron was isolated firstly and then mtRNA was extracted using an improved CTAB method. And finally the higher quality mtRNA was obtained.[Conclusion]The study successfully obtain higher quality mtRNA.

关 键 词：棉花 线粒体RNA提取 方法 

分 类 号：S188[农业科学—农业基础科学]