茅苍术多糖APW1a的分离纯化及结构初步研究  

Separation,Purification and Preliminary Structure Discussion of Polysaccharide APWla from Atractylodes lancea( Thunb. ) DC.

作　　者：段国峰[1] 毛金银[1] 孙如宁[1] 明广奇[1] 欧阳臻[2] 

机构地区：[1]中国药科大学高等职业学院,南京211198 [2]江苏大学药学院,镇江212013

出　　处：《海峡药学》2014年第9期63-65,共3页Strait Pharmaceutical Journal

基　　金：江苏省自然科学基金BK2004062

摘　　要：目的从茅苍术分离纯化出多糖APW1a,研究其组成及初步结构。方法茅苍术根茎经水提醇沉、脱蛋白、透析,DEAE纤维素与Sephadex G-100凝胶柱洗脱得APW1a;采用紫外、高效凝胶液相、气相、红外、核磁共振等色谱分析其组成及初步结构。结果 APW1a由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖组成,物质的量比为0.16∶0.17∶0.24∶0.55∶0.89∶1分子量约为5.3×103D,糖环以β型吡喃糖为主,并有甲基、酯酰等基团。结论分析了茅苍术多糖APW1a的组成及初步结构,为茅苍术多糖的进一步的研究提供了基础。OBJECTIVE To study the isolation ,the composition and preliminary characterization of polysaccha-ride APW1a from Atractylodes lancea（Thunb.） DC.METHODS The crude polysaccharides were obtained by ex-tracting with boiling-water and precipitating with alcohol ,the protein was removed by TCA ,The cleared polysaccha-ride were purified by DEAE-cellulose,Sephadex G-100 column,received APW1a.The composition and characteriza-tion of APW1a was analyzed by UV,HPLC,GC,IR,1H-NMR.RESULTS APW1a consistsed of Rha、Ara、Xyl、Man、Glu、Gal in a molar ratio of 0.16∶0.17∶0.24∶0.55∶0.89∶1.The molecular mass was 5.3 ×10 3 D.The structure was analyzed by chromatography suggesting APW 1a was in the form of pyranose β-glycosidic bond mainly ,and it con-tained methyl group,ester acyl group and so on.CONCLUSION The structure of APW1a is first discussed,these work cuold establish foundation for the further study of Atractylodes Polysaccharides.

关 键 词：茅苍术 多糖 分离纯化 结构研究 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]