益气养阴活血方对糖尿病模型大鼠κ-nephrin蛋白与P22Phox表达的影响  

Effects of Yiqi Yangyin Huoxue Formula on the Expression of κ-nephrin Protein and P22Phox in Diabetes Mellitus Model Rats

作　　者：张春辉[1] 谭忠荣[1] 

机构地区：[1]华中农业大学校医院,武汉430071

出　　处：《中国药房》2014年第39期3662-3664,共3页China Pharmacy

摘　　要：目的:研究益气养阴活血方对糖尿病模型大鼠κ-nephrin蛋白与P22Phox的表达。方法:腹腔注射链脲佐菌素(25 mg/kg)联合高脂饲料以复制大鼠糖尿病模型。60只SD大鼠分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、益气养阴活血方(200 ml/kg)组,灌胃给药,每天2次,连续16周。测定大鼠肾皮质κ-nephrin蛋白与P22Phox的表达。结果:与正常对照组比较,模型组大鼠肾皮质κ-nephrin蛋白表达降低,P22Phox表达增强,差异有统计学意义(P<0.05);与模型组比较,益气养阴活血方组大鼠肾皮质κ-nephrin蛋白表达增强,P22Phox多肽表达减弱,差异有统计学意义(P<0.05)。结论:益气养阴活血方可明显改善糖尿病模型大鼠κ-nephrin蛋白以及P22Phox表达水平。OBJECTIVE：To study the effects of Yiqi yangyin huoxue formula on the expression of nephrin protein and P22 Phox in diabetes mellitus（DM）model rats. METHODS：DM model was induced by intraperitoneal injection of streptozotocin（25 mg/kg）combined with high lipid diet. 60 SD rats were divided intro normal control group（constant volume of normal saline）,model group（constant volume of normal saline）and Yiqi yangyin huoxue formula group（200 ml/kg）. They were given relevant drugs intragastrically twice a day for consecutive 16 weeks. The expression of nephrin protein in renal cortical homogenate of rats and the expression of P22 Phox in cortex renis were all determined by ELISA. RESULTS：Compared with normal control group,the expression of κ-nephrin protein in renal cortical homogenate were decreased,and the expression of P22 Phox in cortex renis were increased in model group;there was statistical significance（P〈0.05）;compared with model group,the expression of κ-nephrin protein in renal cortical homogenate were increased,and the expression of P22 Phox in cortex renis were decreased in Yiqi yangyin huoxue formula;there was statistical significance（P〈0.05）. CONCLUSIONS：Yiqi yangyin huoxue formula can significantly improve the expression of nephrin protein and P22 Phox in DM rats.

关 键 词：益气养阴活血方 κ-nephrin蛋白 P22PHOX 糖尿病 模型 大鼠 

分 类 号：R285[医药卫生—中药学]