HPLC法测定清热地黄酊中毛蕊花糖苷的含量  

Content Determination of Verbascoside in Qingre Dihuang Tincture

作　　者：郭宏彦[1] 涂禾[1] 刘中均 朱力阳[1] 

机构地区：[1]四川省骨科医院,成都610041

出　　处：《中国药房》2014年第39期3703-3704,共2页China Pharmacy

摘　　要：目的：建立测定清热地黄酊中毛蕊花糖苷含量的方法。方法：采用高效液相色谱法。色谱柱为Waters Symmetry Shield C18（250 mm×4.6 mm,5μl）,流动相为乙腈-0.1%醋酸溶液（10∶90,V/V）,检测波长为334 nm,流速为1.0 ml/min,柱温为30℃,进样量为10μl。结果：毛蕊花糖苷质量浓度在187.6-938.0μg/ml范围内与峰面积积分值呈良好的线性关系（r=0.9997）;精密度、稳定性、重复性试验的RSD均≤0.52%;平均加样回收率为99.97%,RSD=0.28%（n=9）。结论：该方法简单、准确、重复性好,可用于清热地黄酊中毛蕊花糖苷的含量测定。OBJECTIVE：To establish the method for the content determination of verbascoside in Qingre dihuang tincture.METHODS：HPLC method was adopted. The determination was performed on Waters Symmetry Shield C18（250 mm×4.6 mm,5 μl）column with mobile phase consisted of acetonitrile-0.1% acetum solution（10∶90,V/V）at the flow rate of 1.0 ml/min. The detection wavelength was set at 334 nm and the column temperature was 30 ℃. The sample size was 10 μl. RESULTS：The linear range of verbascoside was 187.6-938.0 μg/ml（r=0.999 7）. RSDs of precision,stability and reproducibility tests were all lower than0.52% with an average recovery of 99.97%（RSD=0.28%,n=9）. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for content determination of verbascoside in Qingre dihuang tincture.

关 键 词：清热地黄酊 毛蕊花糖苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]