HPLC测定二陈合剂中橙皮苷含量方法研究  

Determination of hesperidine inErchen mixture by HPLC

作　　者：刘菁[1] 何培根[1] 李志浩[1] 李鹏[1] 

机构地区：[1]湖北医药学院附属东风医院药学部,十堰442000

出　　处：《国际中医中药杂志》2014年第10期909-911,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的：建立测定二陈合剂中橙皮苷含量的方法。方法采用高效液相色谱法，色谱柱为Fortis C18（250 mm×4.6 mm，5μm），流动相为乙腈-醋酸-水（22∶4∶74），流速为1.0 ml/min，柱温30℃，检测波长为283 nm。结果橙皮苷在2.86～57.20μg（r＝0.9998）范围有良好的线性关系，平均加样回收率为99.4%；RSD小于2.0%。结论本方法快速、可靠、简便，重复性好，专属性强，可为二陈合剂的质量标准提供参考。Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18（250 mm×4.6 mm,5μm）column and the mobile phase consisted of acetonitrile-acetic acid-water（22∶4∶74）. The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg（r=0.999 8）. The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.

关 键 词：橙皮苷 二陈合剂 高效液相色谱法 

分 类 号：R286[医药卫生—中药学] O657.72[医药卫生—中医学]