A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean(Glycine max(L.) Merri.)  

作　　者：WANG Jun LIU Lin GUO Yong WANG Yong-hui ZHANG Le JIN Long-guo GUAN Rong-xia LIU Zhang-xiong WANG Lin-lin CHANG Ru-zhen QIU Li-juan 

机构地区：[1]National Key Facility for Gene Resources and Genetic Improvement (NFCRI)/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences

出　　处：《Journal of Integrative Agriculture》2014年第9期1854-1864,共11页农业科学学报（英文版）

基　　金：funded by the National High-Tech R&D Program of China(2012AA101106);the National Basic Research Program of China(2009CB118404);the Key Technologies R&D Program of China during the 12th Five-Year Plan period(2011BAD35B06);the National Transgenic Major Program,China(2008ZX08009-003)

摘　　要：Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines（RHL） derived from the cross between Yangyandou（low level of β subunit） and Zhonghuang 13（normal level of β subunit） were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1（β subunit content）, which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome（Glyma 1.0, Williams 82）, including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine（TT） insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection（MAS）.Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines（RHL） derived from the cross between Yangyandou（low level of β subunit） and Zhonghuang 13（normal level of β subunit） were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1（β subunit content）, which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome（Glyma 1.0, Williams 82）, including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine（TT） insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection（MAS）.

关 键 词：soybean seed storage protein β subunit QTL mapping 

分 类 号：S565.1[农业科学—作物学]