高效液相色谱法测定百癣夏塔热片中槲皮素的含量  

Determination of Quercetin in Baixuanxiatare Tablets by HPLC

作　　者：甘书明 王树瑶[2] 

机构地区：[1]黑龙江省济仁药业有限公司,哈尔滨150025 [2]北京中医药大学中药学院,北京100102

出　　处：《黑龙江医药》2014年第5期1020-1022,共3页Heilongjiang Medicine journal

摘　　要：目的:建立HPLC法测定百癣夏塔热片中槲皮素的含量。方法:Diamonsil-C18色谱柱(250mm×4.6mm,5μm);流动相为甲醇-0.3%磷酸溶液(43:57);检测波长为371nm。结果:线性范围为0.0650μg^0.6496μg,平均回收率为99.36%,RSD为0.57%(n=6)。结论:该方法简便,快速,准确可靠。Objective： A HPLC method was established for the determination of quercetin in Baixuanxiatare Tablets. Methods： In the analysis,a Diamonsil-C18（250mm × 4.6mm,5μm） column, a mobile phase of methanol-0.3% Phosphoric acid solution（43：57）and a UV wavelength of 371nm. Results： A linear range of 0.0650μg ~ 0.6496μg,an average recovery of 99.36% with RSD of 0.57%（n=6）. Conclusion： The method of HPLC is simple,rapid and reliable.

关 键 词：高效液相色谱法 槲皮素 百癣夏塔热片 

分 类 号：R927.2[医药卫生—药学]