奥沙利铂对背根神经节神经元凋亡的影响  

Influence of oxaliplatin on apoptosis of dorsal root ganglion neurons

作　　者：苗亚军 王言森[1] 杜向阳[1] 张琼[1] 

机构地区：[1]山东省交通医院,济南250013

出　　处：《山东医药》2014年第22期21-23,共3页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划项目(2013WS0375)

摘　　要：目的观察奥沙利铂(OHP)对体外培养的背根神经节(DRG)神经元的致凋亡作用,并探讨其机制。方法雌性Wistar大鼠雌性20只、雄性5只按4∶1合笼饲养,第2天选取孕鼠,至第12.5天(E12.5)、19.5天(E19.5)时,将所获孕鼠麻醉后取出胚胎,体外培养DRG神经元。将选自E12.5及E19.5的胚胎DRG神经元各分为4组,在E12.5低、中、高剂量组及E12.5对照组培养液中分别加入10、20、50μmol/L OHP及正常细胞培养液;在E19.5低、中、高剂量组及E19.5对照组培养液中分别加入10、20、50μmol/L OHP及正常细胞培养液。各组均作用24 h。采用Hoechst33342染色DRG神经元细胞核,计算凋亡细胞所占比例。采用Western blot法检测凋亡蛋白Caspase-3的表达。结果 E12.5中、高剂量组DRG细胞凋亡比例高于E12.5对照组及E12.5低剂量组(P<0.05)。E12.5中剂量组、E12.5高剂量组Caspase-3蛋白的表达高于E12.5对照组及E12.5低剂量组(P<0.01),而低剂量组与对照组差异无统计学意义。结论 OHP可引起体外培养的E12.5 DRG神经元的凋亡,可能是通过干预DRG神经元的分化发挥致凋亡作用。Objective To investigate if oxaliplatin （ OHP） can induce apoptosis of dorsal root ganglion （ DRG） neurons and the mechanism of OHP-induced apoptosis .Methods In this study, we chose 20 female Wistar rats and 5 male rats, rearing for 4∶1 in five cages, and selected the female rats which had vaginal plug the next day .On embryonic 12.5 day （E12.5） and E19.5, the pregnant rats were anesthetized and then their embryos were removed to culture the DRG neurons in vitro.The cultured embryonic DRG neurons were divided into 4 groups respectively：E12.5 low, medium, high dose groups and E12.5 control group, which were incubated with 10, 20, 50 μmol/L OHP and normal cell culture fluid for 24 hours;E19.5 low, medium, high dose groups and E19.5 control group which were added 10, 20, 50μmol/L OHP and normal cell culture fluid for 24 hours.We used Hoechst33342 to stain the nucleus of DRG neurons and calculated the percentage of apoptosis cells .The expression of caspase-3 protein was detected by Western blotting .Results The percentage of apoptosis cells in the E12.5 medium and high dose groups was higher than that of E 12.5 control and E12.5 low dose groups （P〈0.05）, and there was no significant difference between the low dose group and the control group .The expression of caspase-3 protein in the E12.5 medium dose group and high dose group was higher than that of E 12.5 low dose group and E12.5 control group （P〈0.01）, but there was no significant difference between the low dose group and the control group.Conclusion OHP may induce the apoptosis of DRG neurons cultured in vitro by interfering the differentiation of DRG neurons .

关 键 词：奥沙利铂 背根神经节 神经毒性 凋亡 

分 类 号：R979.1[医药卫生—药品]