HPLC法测定三草胶囊中熊果酸的含量  

Determination of Ursolic acid in Sanchao Capsules by HPLC

作　　者：何丽君[1] 陈豪[1] 郑婉玉[1] 翁淑琴[1] 程心玲[1] 

机构地区：[1]福建中医药大学附属人民医院药学部,福州350004

出　　处：《海峡药学》2014年第8期42-44,共3页Strait Pharmaceutical Journal

基　　金：福建省卫生厅青年科研课题支助项目;项目编号:2008-1-44;福建省科技厅重点项目课题支助项目;项目编号:2014Y01010405

摘　　要：目的建立HPLC法测定三草胶囊中熊果酸的含量。方法采用Acclaim120 C18（5μm,4.6×250mm）色谱柱;流动相为甲醇-0.1%甲酸（88∶12）;流速为1mL·min-1;检测波长为210nm。结果熊果酸在2.98-119.36μg·mL-1范围与峰面积呈良好的线性关系,平均加样回收率为99.2%,RSD%为1.3%。结论该法简便,快速,结果可靠,可用于三草胶囊的质量控制。OBJECTIVE To establish a HPLC method for the determination of Ursolic acid in Sanchao Capsules. METHODS The chromatographic column was Acclaim120 C18（ 5μm,4.6 ×250mm）. The mobile phase was Methyl alcohol-formic acid（ 88 ∶ 12）. The flow rate was 1mL · min- 1and the detection wavelength was210 nm. RESULTS The standard curves for Ursolic acid was liner in the rang of 2. 98 ~ 119. 36μg·mL- 1,The average recovey was ranged from 99. 2%,RSD% was 1. 3%. CONCLUSION This method is simple,quick and believable. It can be effectively used for the quality control of Sanchao Capsules.

关 键 词：高效液相色谱法 三草胶囊 熊果酸 

分 类 号：R927.2[医药卫生—药学]