基于过氧化物模拟酶催化显色反应的博来霉素比色检测  

Bleomycin·Fe(Ⅱ) Complex with Intrinsic Peroxidase-like Activity for Colorimetric Detection of Bleomycin

作　　者：芦珊[1] 杨媚[2] 王海燕[3] 曹亚[2] 殷咏梅[1] 

机构地区：[1]南京医科大学第一附属医院肿瘤科,江苏南京210029 [2]上海大学生命科学学院生物传感实验室,上海200444 [3]南京大学生物化学系,江苏南京210093

出　　处：《分析测试学报》2014年第9期1068-1072,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(81172503)

摘　　要：首次报道了博来霉素(BLM)与亚铁离子相互结合,形成的BLM·Fe(Ⅱ)复合物具有内在的过氧化物模拟酶催化活性,能够催化过氧化氢氧化2,2'-联氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)的显色反应,产生深绿色的产物。与天然酶辣根过氧化物酶类似,BLM·Fe(Ⅱ)复合物的催化活性强烈依赖于pH值和温度,相应的最优化条件分别为pH 6.0和30℃。利用BLM·Fe(Ⅱ)复合物催化ABTS的显色反应,建立了一种简便快捷的可视化检测博来霉素的新方法,检出限可达14.1 nmol/L。该检测方法还显示了良好的可重复性和选择性,在临床分析中具有潜在的应用价值。Bleomycins （BLMs）, a family of glycopeptide-derived antibiotics, are widely used as available chemotherapeutic agents against a variety of cancers. In the presence of Fe（ U ） ions, BLM form a binary BLM · Fe（ Ⅱ ）complex that can activate molecular oxygen. Herein, it is demonstrate for the first time that BLM · Fe （Ⅱ ） complex may possess an intrinsic peroxidase-like activity and could catalyze 2, 2＇-azino-bis（3-ethylbenzthiazoline-6-sulfonic acid） （ABTS） into colored product in the presence of hydrogen peroxide. Experimental results revealed that the catalytic activity of BLM · Fe（ Ⅱ ） complex is strongly dependdnt on pH value and temperature, which is similar to natural enzymes such as horseradish peroxidase. The conditions for maximum catalytic efficiency were determined to be pH 6.0 and 30 ℃. Taking advantage of the color reaction of ABTS catalyzed by BLM · Fe（ Ⅱ ） complex, a simple and rapid colorimetric method for the detection of BLM was thus established. Under the optimal conditions, a good linear relationship was obtained over BLM concentration in the range of 0.1 -2.5 μmol/L with a detection limit of 14. 1 nmol/L. With the advantages of good reproducibility and high selectivity, the method is anticipated to have a practical potential for

关 键 词：博来霉素 过氧化物模拟酶 紫外-可见吸收光谱 比色检测 

分 类 号：O657.72[理学—分析化学] R978.1[理学—化学]