胰岛素样生长因子结合蛋白5基因在绒山羊染色体的定位研究  

Study on the Positioning for Insulin-like Growth Factor Binding Protein 5 Gene (IGFBP-5) in Cashmere Goat Chromosome

作　　者：刘志红[1] 于新蕾[1] 俎红丽[1] 蔡婷[1] 王志新[1] 高丽霞[1] 李金泉[1] 肖红梅[2] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 [2]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《中国畜牧杂志》2014年第19期16-19,共4页Chinese Journal of Animal Science

基　　金：国家高技术研究发展计划项目(863计划)(2013AA102506);国家自然科学基金项目(31272421;31360537;31072005);国家农业部现代农业产业技术体系项目(CARS-40-05)

摘　　要：胰岛素样生长因子结合蛋白5(IGFBP-5)是胰岛素样生长因子IGFS的重要调控者,在毛囊细胞的发育和生长周期中起着重要的调控作用。为丰富绒山羊基因组物理图谱和更好地研究绒毛调控机理,本实验利用荧光原位杂交(FISH)技术,以提取的IGFBP-5BAC质粒作为探针与公绒山羊染色体核型标本进行杂交,将IGFBP-5基因定位于绒山羊7号染色体的长臂第一区段第5区。该研究首次报道了山羊IGFBP-5基因的染色体定位,完善了山羊染色体物理图谱和山羊基因组信息。Insulin-like growth factor bind ing protein 5 gene（IGFBP-5） is an important regulator for insulin-like growth factor（IGFS）, and plays a significant role in the development and cyclic growth of hair follicle. In order to enrich goat genome physical map and better study the regulation mechanism of Cashmere, the extracted IGFBP-5BAC plasmid as a probe were hybridized with karyotype samples of male goat by fluorescence in situ hybridization（FISH）,as a result, IGFBP-5 gene was located on chromosome 7 of the long arm of the 5th district. The study firstly reported that localization of IGFBP-5 gene in goat. It improved the physical map and the genomic information in goat.

关 键 词：IGFBP-5 染色体定位 荧光原位杂交 绒山羊 

分 类 号：S827.2[农业科学—畜牧学]