影响山羊体内原核胚生产的因素研究  

Factors That Influence in vivo Production of Goat Pronuclear-stage Embryo

作　　者：张航[1,2] 秦文向 任康 宋玉坤[2] 常连忠 姚玉昌[5] 连正兴[2] 吕文发[1] 刘国世[2] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]中国农业大学动物科技学院,北京100193 [3]北京市畜牧总站,北京100107 [4]青岛森淼实业有限公司,山东青岛266000 [5]东北农业大学,黑龙江哈尔滨150030

出　　处：《中国畜牧杂志》2014年第19期28-32,共5页Chinese Journal of Animal Science

基　　金：转基因重大专项(2014ZX0800802B;2011ZX08008-005);国家科技支撑项目(2011BAD19B02-4)

摘　　要：为提高山羊原核期胚胎生产效率,实验选取107只供体山羊（1～5岁）进行超数排卵并收集原核胚胎,探索山东地区不同山羊品种（嘉祥羊和沂水羊）、季节、体重、供体山羊使用次数、超数排卵方案对超数排卵及冲胚效果的影响。结果表明：嘉祥羊、沂水羊2个品种原核胚生产效率相似;春季与秋季山羊平均冲胚数、可用胚数、移植妊娠率无显著差异（P〉0.05）;体重40 kg以上的羊冲胚效果显著高于30～40 kg的供体山羊（P〈0.05）;供体山羊手术次数大于3次时,获得的可用胚胎数开始下降,5～6次最低达到显著差异（P〈0.05）;CIDR＋FSH慢减量4 d 8针注射法在平均黄体个数、平均冲胚数和平均可用胚数方面均显著高于CIDR＋FSH减量4 d 8针注射法（P〈0.05）。综上所述,选择手术次数小于等于3次,体重40 kg以上的山羊作为供体,采用CIDR＋FSH慢减量4 d 8针注射法的超排方案可以提高原核胚生产效率。The objective of this study was to improve the efficiency of pronuclear-stage embryo yield to be used forDNA microinjection in a transgenesis goat program. A total of 107 goats with different body weight and different breeds in Shangdong porvince were selected as donors（1 ～5 years old）for superovulation and pronuclear embryo collection using different superovulation programs, sexual mating and embryo flushing in spring and winter. The results showed that the efficiency of pronuclear-stage embryo yields between Jia Xiang and Yi Shui goat breeds was similar. The average numer of embryos recovered per goat with over 40 kg body weight was significantly higher than that of with30 ～40 kg body weight（P〈0.05）. When the donor＇s surgery number was over 3 times, the number of available embryos decreased and came to the bottom at 5～6 times surgery with significant difference（P〈0.05）. There was no significnta difference between spring and autumn in the average number of recovered embryos, of embryos used for transfer and the pregnancy rate of goats（P〉0.05）. The average number of pronuclear-stage embryos availabe for transfer was higher using the FSH-8-injection slowing reduction than FSH-8-injection reduction. The data demonstrated that the efficiency of pronuclear-stage embryo yield could be improved when the goats with more than50 kg body weight and less than 3-times surgery were selected.

关 键 词：原核胚 超数排卵 山羊 

分 类 号：S827.3[农业科学—畜牧学]