地黄和小肽对鲤鱼生长性能及IL-8表达的调节  

Effects of Rehmannia glutinosa and Small Peptide on Growth and Expression of Interleukin-8in Common Carp Cyprinus carpio

作　　者：王俊丽[1] 雒燕婷[1] 郝光[1] 闫潇[2] 吴春[2] 聂国兴[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南师范大学水产学院,河南新乡453007

出　　处：《水产科学》2014年第9期556-561,共6页Fisheries Science

基　　金：河南省高校科技创新团队支持计划(14IRTSTHN013);河南省教育厅科学技术研究重点项目(12B240002)

摘　　要：研究了地黄和深海鱼源活性小肽对鲤鱼生长和免疫功能的调节作用,探讨其作为鱼类饲料添加剂的可行性。试验一选取540尾平均体质量为(7.46±1.06)g的鲤鱼,随机分为9组饲喂基础日粮及添加了2%生地黄粉,4%生地黄粉,2%熟地黄粉,4%熟地黄粉,0.5%生地黄水提物,1%生地黄水提物,0.5%熟地黄水提物,1%熟地黄水提物的饲料。试验二选取360尾平均体质量为(5.47±0.3)g的鲤鱼,随机分为4组饲喂基础日粮和添加5%、10%和15%小肽的饲料。饲养80d后,测定鲤鱼的生长指标和抗病性能,并检测鲤鱼脾脏IL-8的mRNA表达情况。结果显示,各种地黄制剂和不同剂量的小肽均可提高鲤鱼脾脏IL-8的表达,其中试验一的2%熟地黄粉、4%熟地黄粉、1%生地黄水提物、0.5%熟地黄水提物组和试验二的5%、10%小肽组与对照组均有显著性差异。地黄还可显著促进鲤的生长,提高其抗病力。因此,地黄和深海鱼源活性小肽均有望成为具有免疫调节作用的鱼类饲料添加剂。The effects of Rehmannia glutinosaand marine source bioactive small peptide on growth and immune function in common carp（Cyprinus carpio）were investigated to develop Rehmannia glutinosa and the small peptide as fish feed additives.In Experiment 1,540 common carp juveniles with body weight of（7.46±1.06）g were randomly allocated into nine groups,and fed basal diet（control group）,the diets containing radix rehmanniae powder at a dose of 2%and 4%,the diets containing prepared rehmannia root powder at a dose of 2%and 4%,the diets containing radix rehmanniae extract at a rate of 0.5%,and 1%,or the diets containing prepared rehmannia root extract at a dose of 0.5%and 1%for 80 days.In Experiment 2,360 common carp juveniles with body weight of（5.47±0.3）g were randomly allocated into four groups,and fed the basal diet（control group）,and the diets containing small peptide at a rate of 5%,10%and 15%for 80 days.At the end of the experiments,the growth performance,disease resistance and the IL-8（interleukin-8）mRNA expression in spleen were studied in the juveniles.The results showed that the higher expression of IL-8in the spleen was found in the juveniles fed the diets containing all sorts of rehmannia preparations and different doses of the small peptide,significantly higher in 2%prepared rehmannia root powder,4%prepared rehmannia root powder,1%radix rehmanniae extract,0.5%prepared rehmannia root extract and 5%and 10%small peptide groups than those in the control groups,indicating that Rehmannia glutinosacan significantly promote the growth and disease resistance of common carp.Therefore,Rehmannia glutinosa and marine source bioactive peptide are expected to become good fish feed additives.

关 键 词：地黄 小肽 鲤鱼 生长性能 IL-8 

分 类 号：S965.116[农业科学—水产养殖]