海口地区住院老年病人口腔假丝酵母菌种类分布研究  

Distribution of oral Candida in elderly patients of Haikou city

作　　者：贝筝 贝宁[1,2] 吴亚丹 符秀梅[1,2] 龙登毅 王英[3] 王华民 

机构地区：[1]海南省老年病医院 [2]海南省老年病医院热带老年病研究所,海南海口571100 [3]海南医学院热带医学与检验医学院,海南海口571199

出　　处：《中国热带医学》2014年第9期1080-1082,共3页China Tropical Medicine

基　　金：国家自然科学基金(No.30760001)

摘　　要：目的研究住院老年病人口腔假丝酵母菌的携带分布情况及抗生素用药对其携带的影响。方法将来源于医院住院病人的口腔拭子先保存在含有氯霉素的YEPD(Yeast extract peptone dextrose)液体培养基中,然后划线接种于YEPD平板上37℃培养,通过观察菌落形态、涂片乳酸酚棉蓝染色、科玛嘉显色培养基培养,芽管形成试验、厚膜孢子及假菌丝生成试验等对菌种进行鉴定。结果共采集住院老年病人口腔拭子标本403例,假丝酵母菌总分离率为62.78%(253/403),其中白假丝酵母菌150株,占59.29%,光滑假丝酵母菌72株,占28.46%,热带假丝酵母菌5株,占1.98%,其他假丝酵母菌26株,占10.27%。不同性别患者的假丝酵母菌分离率差异无统计学意义,P>0.05,应用抗生素与未应用抗生素患者的假丝酵母菌的分离率差异具有统计学意义,P<0.05。结论住院老年病人口腔中假丝酵母菌出现明显高的分离率,且以白假丝酵母菌为最多,其次是光滑假丝酵母菌。Objective To survey the distribution of oral Candida species in inpatients and the use of antibiotics for treatment of the infections. Methods The swabs collected were cultured in yeast extract peptone dextrose and the candida stained with lactic acid phenol cotton blue was morphologically identified by germ tube, chlamydospore test and chromagarmedium. Results The detectable rate of Candida in the oral cavity of inpatients was 62.78% （253/403）, including 150 C. albieans strains （59.29%）;72 C. glabrata strains （28.46%）; 5 C. tropiealis strains （1.98%） and 26 other Candida strains （10.27%）. Separation of different gender patients with candida no statistically significant difference, P〉 0.05. Use of antibiotics and the nonuse of antibiotics in patients with candida separation rate difference is statistically significant, P 〈 0.05. Conclusion The detectable rate of Candida in the oral cavity of inpatients is higher than healthy population and Candida albieans predominated, followed by C. glabrata.

关 键 词：假丝酵母菌 分布 口腔 住院病人 

分 类 号：R37[医药卫生—病原生物学]