流动注射聚离子敏感膜电极检测三磷酸腺苷  

Flow Injection Biosensors for Determination of ATP Based on Polyion-Sensitive Membrane Electrode

作　　者：雷佳宏 丁家旺[1] 秦伟[1] 

机构地区：[1]海岸带环境过程与生态修复重点实验室中国科学院烟台海岸带研究所,山东烟台264000 [2]中国科学院大学,北京100049

出　　处：《化学与生物工程》2014年第9期71-75,共5页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(41176081;21207156)

摘　　要：以核酸适体为识别分子、以经鱼精蛋白活化的聚离子敏感膜电极为信号识别单元,构建了一种检测三磷酸腺苷（ATP）的流动注射分析方法。核酸适体与鱼精蛋白之间的静电作用能够引起电极电位的变化,核酸适体与ATP的相互作用使核酸适体的形态发生一定的变化,这种变化能够阻止核酸适体与鱼精蛋白之间的作用,据此实现对ATP的电位检测。结果表明,在电流为10nA、载液流速为2.0mL·min-1、进样量为80μL的优化条件下,本方法对ATP检测的线性范围为2.0~12.0μmol·L-1,检出限为1.4μmol·L-1。本方法亦可用于对其它聚阴离子检测。A flow injection analysis（FIA）system for determination of adenosine triphosphate（ATP）was developed.The polyion-sensitive membrane electrodes were used for potentiometric detection and the aptamers were used for molecular recognition.The electrostatic interaction between aptamer and protamine could cause potential response on the electrodes.Interaction between aptamer and ATP changed the forms of aptamer,which prevented the interaction between aptamer and protamine.Based on that,the concentration of ATP could be determined by potential detection.Results showed that,under optimized conditions（current of 10 nA,flow rate of2.0mL·min-1,sample volume of 80μL）,the proposed method showed a good linear relationship to ATP in the concentration range from 2.0to 12.0μmol·L-1.The detection limit was calculated to be 1.4μmol·L-1.This method can also be used for the determination of other targets.

关 键 词：生物传感器 三磷酸腺苷(ATP) 流动注射分析 核酸适体 聚离子敏感膜电极 

分 类 号：O657.99[理学—分析化学]