固定化Pseudomonas putida CGMCC3830转化3-氰基吡啶制备烟酸  

Biotransformation of 3-Cyanopyridine to Nicotinic Acid with Immobilized Pseudomonas putida CGMCC3830

作　　者：李恒[1] 赵欣[1] 杨涛[1] 龚劲松[1] 朱小燕[1] 熊雷[1] 陆震鸣[1] 许正宏[1] 史劲松[1] 

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《食品与生物技术学报》2014年第8期800-804,共5页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2012AA022204C);国家自然基金项目(21206055);江苏省自然科学基金项目(BK2012127)

摘　　要：采用固定化Pseudomonas putida CGMCC3830转化3-氰基吡啶制备烟酸。选择海藻酸钠作为包埋材料进行固定化,考察固定化细胞的制备条件、转化条件以及批次稳定性。结果表明,优化的固定化条件为:海藻酸钠2 g/dL,氯化钙0.4 g/dL,固化时间6 h;最适转化条件为:温度35℃,pH 7.0,底物浓度100 mmol/L。批次转化实验结果显示,当底物浓度为100 mmol/L时,固定化细胞重复使用10次,酶活仍保留59.1%,产物烟酸的得率为91.8 g/g细胞干重,而游离细胞的使用3次后,酶活下降至45.6%。Biotransformation of 3-cyanopyridine to nicotinic acid with immobilized Pseudomonas putida CGMCC3830 was studied.Sodium alginate was selected as the immobilization material.The cell immobilization conditions,biotransformation conditions and the batch stability were investigated.The optimized conditions for immobilization were as follows：sodium alginate 2 g/dL,calcium chloride 0.4 g/dL,curing time 6 h.The optimized immobilization conditions included：temperature 35 ℃,pH 7.0,substrate concentration 100 mmol/L.Results of batch conversion experiments showed that with the substrate concentration of 100 mmol/L,the nicotinic acid yield reached 91.8 g/g cell dry weight.The immobilized cell can be reused 10 times with the enzymatic activity retained 59.1％,while the free cell could be reused only 3 times with the enzymatic activity retained 45.6％.

关 键 词：Pseudomonas PUTIDA 固定化 烟酸 3-氰基吡啶 腈水解酶 

分 类 号：TQ466.2[化学工程—制药化工]