Integrated solid-state nanopore devices for third generation DNA sequencing  

作　　者：WU GenSheng ZHANG Yin SI Wei SHA JingJie LIU Lei CHEN YunFei 

机构地区：[1]Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University

出　　处：《Science China(Technological Sciences)》2014年第10期1925-1935,共11页中国科学（技术科学英文版）

基　　金：supported by the National Basic Research Program of China(Grant No.2011CB707605);National Natural Science Foundation of China(Grant Nos.50925519 and 51375092);G.S.Wu was supported by the Scientific Research Foundation of Graduate School of Southeast University

摘　　要：Third generation DNA sequencing relies on monitoring the ionic current blockage during the DNA molecule’s threading through a nanoscale pore.It is still really tough to attain the single base discrimination on a DNA strand by merely analyzing the ionic current due to speedy DNA translocation and low spatial resolution.More integrated configurations are pursued to present versatile comparative dissimilarities of the four bases by enhancing the spatial resolution within a DNA molecule translocation event,such as transverse tunneling current,local potential change,and capacitance oriented voltage resonance.In this mini review,the insight is provided into the status quo on several functionalized techniques and methodologies for DNA sequencing and furthermore concluding remark and outlook are presented.

关 键 词：DNA sequencing solid-state nanopore spatial resolution integrated sensors 

分 类 号：Q503[生物学—生物化学]