缺氧诱导因子-1_α和基质蛋白酶-9在鼻息肉嗜酸性粒细胞浸润过程中的作用  

Effect of HIF-1αand MMP-9in the infiltration of eosinophile granulocyte in nasalpolyps

作　　者：佘腊枝[1] 鲁海涛[1] 谢琼[1] 

机构地区：[1]湖北省荆州市中心医院耳鼻喉科,434020

出　　处：《检验医学与临床》2014年第18期2553-2554,2556,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨缺氧诱导因子-1α(HIF-1α)和基质金属蛋白酶-9(MMP-9)在鼻息肉组织中的表达及其与嗜酸性粒细胞(EOS)浸润的关系。方法于2010年10月至2012年12月,以31例经鼻内窥镜手术治疗的鼻息肉患者作为实验组,以11例行下鼻甲部分切除术治疗的慢性肥厚性鼻炎患者作为对照组,分别采集鼻息肉组织和下鼻甲黏膜组织,检测并分析组织中HIF-1α、MMP-9mRNA表达水平,以及HIF-1α和MMP-9阳性细胞数、阳性血管数和EOS浸润数。结果观察组HIF-1α和MMP-9mRNA表达水平明显高于对照组(P<0.05)。观察组HIF-1α和MMP-9阳性细胞数、阳性血管数和EOS浸润数均高于对照组(P<0.05)。观察组HIF-1α和MMP-9阳性细胞数与EOS浸润数均呈正相关(相关系数分别为0.56、0.88,P<0.05)。结论 HIF-1α和MMP-9在鼻息肉组织中呈高水平表达,二者可能在鼻息肉在发病机制中具有重要作用。Objective To investigate the expressions of hypoxia induced factor-lα（HIF-1α）and matrix metalloproteinase-9（MMP-9）in nasalpolyps tissues and their relationships with the infiltration of eosinophilic granulocyte（EOS）in nasalpolyps tissues.Methods During Oct.2010 and Dec.2012,nasal polyp tissues were collected from 31 patients with nasalpolyps treated with nasal endoscopy（observation group）,and inferior turbinate mucosa were collected from 11 patients with chronic hypertrophic rhinitis treated with partial inferior turbinectomy（control group）.mRNA expression levels of HIF-1αand MMP-9in tissue samples of the two groups were assayed.Amounts of cells and blood vessels positive with HIF-1αand MMP-9,and infiltration amounts of EOS were also detected.ResultsmRNA expression levels of HIF-1αand MMP-9in observation group were higher than control group（P〈0.05）.The amounts of cells and blood vessels positive with HIF-1αand MMP-9and the infiltration amounts of EOS in observation group were also higher than control group（P〈0.05）.In observation group,the amounts of cells positive with HIF-1αand MMP-9were positively correlated with the infiltration amounts of EOS（with correlation coefficients of0.56 and 0.88 respectively,P〈0.05）.Conclusion Expression levels of HIF-1αand MMP-9could be high in nasalpolyps tissues,which might be closely related with the pathogenesis of nasalpolyps.

关 键 词：鼻息肉 缺氧诱导因子-1Α 基质金属蛋白酶-9 嗜酸性粒细胞 

分 类 号：R732.2[医药卫生—肿瘤]