高强度运动训练对大鼠骨骼肌细胞IL-6表达的影响  

Effect of high intensive exercise training on IL-6 expression in skeletal muscle cells of rats

作　　者：王成艳[1] 叶路[2] 李覃[2] 王翼腾 

机构地区：[1]中国人民武装警察部队后勤学院科研部中心实验室,天津 300309 [2]中国人民武装警察部队后勤学院临床医学系病原生物与免疫学教研室, 天津300309 [3]中国人民武装警察部队后勤学院研究生大队, 天津300309

出　　处：《中国急救复苏与灾害医学杂志》2014年第9期830-832,共3页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：中国人民武装警察部队后勤学院科研项目（WYM-201208）

摘　　要：目的：研究高强度运动训练条件对大鼠骨骼肌细胞IL-6表达水平的影响。方法首先建立高强度运动训练大鼠动物模型，然后采用双抗体夹心ELISA法，检测高强度运动训练后大鼠外周血以及骨骼肌细胞IL-6的表达水平。结果经高强度运动训练后，大鼠骨骼肌细胞及外周血IL-6含量均有所升高。结果显示；经过高强度运动后，大鼠血清中IL-6的含量与对照组相比有明显升高，各组结果差异有统计学意义，P〈0.01，高强度运动组大鼠骨骼肌细胞中IL-6的含量与对照组相比，结果差异有统计学意义，P〈0.05。但是一次性力竭运动组大鼠外周血及骨骼肌细胞中IL-6的含量与对照组相比，结果差异无统计学意义，P＆gt;0.05。结论高强度运动训练可以诱导大鼠骨骼肌细胞及外周血中IL-6的表达，发挥抗炎作用。Objective To investigate the effect of high intensive exercise training on IL-6 expression in skeletal muscle cells of rats. Methods Double-antibody sandwich ELISA was used to measure the level of IL-6 in between the experiment group （high intensive exercise training） and the control group. Results The level of IL-6 in serum and skeletal muscle cells of rats increased when the experiment group was trained for high intensive exercise. The results showed that the content of IL-6 in serum of the experiment group was significantly higher than that of control group ,P〈0.01. Meanwhile, the content of IL-6 in skeletal muscle cells of the experiment group was increased obviously in comparing with the control group ,P〈0.05. However, the content of IL-6 reflected no significant differences between one bout exhaust exercise group and control group , P＆gt;0.05. Conclusion High intensive exercise training may promote the expression of IL-6 in serum and skeletal muscle cells of rats, which would be beneficial for treating inflammation.

关 键 词：运动训练 大鼠 骨骼肌 白细胞介素-6 IL-6 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]