限食对衰老大鼠心功能的保护作用及其机制  

Protective effect of dietary restriction on cardiac function in aging rats

作　　者：蒋荣[1] 侯文婷[1] 刘雪姣[1] 申芳[1] 窦爽 冷一平[1] 

机构地区：[1]长沙医学院药理教研室,410219

出　　处：《中华老年心脑血管病杂志》2014年第9期964-966,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：湖南省普通高校教学改革研究项目[(2011)315];湖南省大学生研究性学习和创新性实验计划项目[(2011)272]

摘　　要：目的研究限食对衰老大鼠心功能的保护作用及其机制。方法选择2月龄SD大鼠10只,随机分为衰老组和限食组(60%进食量),每组5只。2组大鼠每天皮下注射D-半乳糖100mg/kg,连续给药42d建立衰老动物模型;衰老模型建立后另选5只2月龄SD大鼠设为青年组。3组行心功能检测,包括左心室内压(LVSP)、左心室舒张末压(LVEDP)、左心室压力最大上升速率(+dp/dtmax),以及血浆丙二醛水平、超氧化物歧化酶活性和左心室心肌脂褐素水平测定。结果与青年组比较,衰老组大鼠LVSP、+dp/dtmax、超氧化物歧化酶活性明显降低,LVEDP、丙二醛、脂褐素水平明显升高,差异有统计学意义(P<0.05,P<0.01);与衰老组比较,限食组LVSP[(110.88±7.35)mm Hg(1mm Hg=0.133kPa)vs(70.18±19.27)mm Hg]、+dp/dtmax[(2827.60±237.88)mm Hg/s vs(2365.66±99.81)mm Hg/s]和超氧化物歧化酶[(115.77±10.17)U/ml vs(90.10±17.11)U/ml]活性明显升高,LVEDP[(7.12±2.51)mm Hg vs(14.05±2.01)mm Hg]、丙二醛[(12.54±1.66)nmol/ml vs(15.83±2.51)nmol/ml]和脂褐素[(348.82±27.29)ng/mg vs(400.12±31.89)ng/mg]水平明显降低,差异有统计学意义(P<0.05,P<0.01)。结论衰老大鼠心功能有所降低,限食可能通过降低机体氧化应激水平,使脂褐素形成减少,从而改善衰老大鼠心功能。Objective To study the protective effect of dietary restriction on cardiac function in aging rats.Methods Ten SD rats aged 2months were randomly divided into aging group and dietary restriction（60%food intake）group（5in each group）.An aging rat model was established by daily subcutaneous injection of D-galactose（100mg/kg）for 42 days.Another 5SD rats aged 2months served as an adolecent group.The cardiac functions（including LVSP,LVEDP,＋dp/dtmax）were detected and the plasma MDA level,SOD activity,left ventricular myocardial lipofuscin level were measured in 3groups.Results The LVSP,＋dp/dtmax,SOD activity were significantly lower while the LVEDP,plasma MDA level,myocardial lipofuscin level were significantly higher in aging group than in adolecent group（P〈0.05,P〈0.01）.The LVSP,＋dp/dtmax,SOD activity were significantly higher while the LVEDP,plasma MDA level,myocardial lipofuscin level were significantly lower in dietary restriction group than in aging group（110.88±7.35 mm Hg vs 70.18±19.27 mm Hg,2827.60±237.88 mm Hg/s vs 2365.66±99.81 mm Hg/s,115.77±10.17U/ml vs 90.10±17.11U/ml,7.12±2.51 mm Hg vs 14.05±2.01 mm Hg,12.54±1.66nmol/ml vs 15.83±2.51nmol/ml,348.82±27.29ng/mg vs 400.12±31.89ng/mg,P〈0.05,P〈0.01）.Conclusion The cardiac function decreases in aging rats.Dietary restriction can imporve their cardiac function by reducing the oxidative stress level and lipofuscin formation.

关 键 词：氧化性应激 脂褐素 丙二醛 超氧化物歧化酶 细胞衰老 

分 类 号：R542.2[医药卫生—心血管疾病]