海藻玉壶汤加减方对实验性自身免疫性甲状腺炎凋亡蛋白Fas/FasL表达的影响  

Effect of Modified Haizao Yuhu Decoction on Expression of Fas/FasL in Experimental Autoimmune Thyroiditis Rats

作　　者：贾燕丽 田港[1] 唐晓霞[1] 张小燕[1] 宋霄宏[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2014年第10期2456-2458,I0016,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(Y2110967);浙江省大学生科技创新活动计划暨新苗人才计划项目(2012R410058)

摘　　要：目的:观察海藻玉壶汤加减方对实验性自身免疫性甲状腺炎(EAT)大鼠甲状腺中Fas/FasL蛋白表达的影响。方法:在建立EAT大鼠模型的基础上,设立海藻玉壶汤加减方(浸膏)高、低剂量组、模型组、正常组、丙基硫氧嘧啶组、雷公藤多苷片组、夏枯草膏组,采用免疫组化法检测EAT大鼠的Fas/FasL蛋白表达情况。结果:正常组甲状腺组织Fas和FasL免疫阳性物表达颜色较浅,表达范围小,模型组免疫阳性物颜色较深,表达范围广。其中,模型组的Fas和FasL表达程度与正常组相比有显著性差异(P<0.01);海藻玉壶汤加减方高剂量组、雷公藤多苷片组与丙基硫氧嘧啶组、夏枯草膏组相比有显著性差异(P<0.01)。结论:海藻玉壶汤加减方高剂量组对凋亡蛋白Fas/FasL的表达具有一定的抑制作用,提示海藻玉壶汤加减方可能通过抑制各凋亡蛋白的表达,从而避免细胞过度凋亡对甲状腺组织的破坏。Objective： To observe the effect of Modified Haizao Yuhu Decoction on the expression of Fas /FasL in thyroid function and auto- antibody in experimental autoimmune thyroiditis（ EAT） rat. Methods： After rat models of EAT were established,rat models of EAT were divided into 7 groups and treated with the extract of Modified Haizao Yuhu Decoction group（ low- does,high- does）,model group,Propylthiouracil tablets group,Spica prunellae ointment group,Tripterygium Glycosides group and normal group. After treatment,Fas /FasL in serum of rat was measured by immunohistochemistry. Results：Fas /FasL immune positive substance of normal group presented light color,the expression ranges were very small. Fas /FasL immune positive substance of model group presented high concentration and the expression ranges were extent. The expression of Fas /FasL in thyroid cells of model group was significantly higher than that in the normal group（P〈0. 01）. Modified Haizao Yuhu Decoction group（high- does） and Tripterygium Glycosides group had significant difference compared with ropylthiouracil tablets group and Spica prunellae ointment group（ P〈0. 01）. Conclusion：The expressions of Fas /FasL were significant lower in EAT rats treated with the Modified Haizao Yuhu Decoction group（high-does）. Modified Haizao Yuhu Decoction can effectively restrain the apoptosis thyroid epithelial cells.

关 键 词：海藻玉壶汤加减方 实验性自身免疫性甲状腺炎 FAS FASL 免疫组化 

分 类 号：R285.5[医药卫生—中药学]