UPLC法同时测定桑黄中Inoscavin A和Hypholomine B的含量  

Simultaneous Determination of Inoscavin A and Hypholomine B in Phellinus spp.by UPLC

作　　者：董宇[1,2] 陈莉君[3] 李洪玉[1,2] 俞忠明[1,2] 费莹[3] 马相锋 寿旦[1,2] 

机构地区：[1]浙江省中医药研究院,浙江杭州310007 [2]浙江省中西医结合肿瘤诊治重点实验室,浙江杭州310007 [3]浙江中医药大学,浙江杭州310053 [4]杭州清正生物技术有限公司,浙江杭州311700

出　　处：《中华中医药学刊》2014年第10期2483-2485,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅公益性技术应用研究项目(2012C33125);浙江省科技厅科技公共服务项目(2012F30027);浙江省中医药科技计划项目(2013ZQ002)

摘　　要：目的：采用超高效液相色谱法（UPLC）,建立同时测定桑黄中Inoscavin A和Hypholomine B含量的方法,并进行鉴别与评价应用。方法：采用Waters ACQUITY UPLC BEH C18色谱柱（2.1 mm×100 mm,1.7μm）;流动相：0.1%甲酸水-0.1%甲酸乙腈进行梯度洗脱;检测波长：395 nm;流速：0.3 mL/min;柱温：35℃;进样体积：2.5μL。结果：Inoscavin A和Hypholomine B分别在0.0015～0.036 mg/mL和0.0075～0.118 mg/mL的范围内具有良好的线性关系,平均回收率分别为93.6%和96.5%。不同种属及产地桑黄中Inoscavin A和Hypholomine B的含量存在显著差异,其中以浙江淳安的火木针层孔菌桑黄中两者的含量最高。结论：该定量测定方法稳定、简便、快速,可应用于桑黄的种属鉴别和质量评价。Objective： To establish the method to determine and compare the contents of Inoscavin A and Hypholomine B in different Phellinus spp. by UPLC. Method： The method by UPLC used a Waters ACQUITY UPLC BEH C18column（2.1 mm ×100 mm,1.7 μm） with the mobile phase of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid with the linear gradient elution. The flow rate was 0. 3 mL /min and the wavelength was 395 nm,with a injection volume in 2. 5 μL,the whole process was under the column temperature of 35 ℃. Results： Inoscavin A and Hypholomine B showed a good linearity in 0. 0015- 0. 036 mg /mL and 0. 0075- 0. 118 mg /mL,respectively. The average recoveries of the two compounds were 93. 6% and 96. 5%,respectively. Conclusion： A significant difference was observed in the comparison with different Phellinus spp. by UPLC. Phellinus igniarius from Zhejiang Qiandaohu showed the highest contain of Inoscavin A and Hypholomine B. The method was simple,rapid and accurate to be used in species identification and quality evaluation of Phellinus spp..

关 键 词：UPLC 桑黄 Inoscavin A Hypholomine B 

分 类 号：R284[医药卫生—中药学]