麝香保心丸促进血管新生作用的活性成分筛选  

Screening of active compounds of pro-angiogenic in Shexiang Baoxin pill

作　　者：吕超[1] 黄慧梅[1] 畅婉琳[1] 柳润辉[1] 

机构地区：[1]福建中医药大学药学院,福建福州350108

出　　处：《药学实践杂志》2014年第5期344-347,351,共5页Journal of Pharmaceutical Practice

摘　　要：目的对麝香保心丸中单体入血促进血管新生活性成分进行筛选,以确定其中发挥促进血管新生作用的药效物质。方法采用实时细胞分析仪（xCELLigence系统）检测麝香保心丸及其单体入血成分对内皮细胞增殖、迁移活性的影响,并建立细胞体外成管模型和大鼠主动脉环模型评价麝香保心丸及其入血单体成分的体外血管新生活性。结果细胞增殖、迁移及体外成管实验结果显示,不同浓度的麝香保心丸（10^-4-10^-2μg/ml）、人参皂苷Rg3（1-10μmol/L）和人参皂苷Rh2（1-10μmol/L）能够明显促进人脐静脉内皮细胞（HUVECs）增殖、迁移及管腔结构形成（P〈0.05）。此外,与对照组相比,高浓度麝香保心丸（10-2μg/ml）、Rg3（10μmol/L）和Rh2（10μmol/L）具有诱导主动脉环内皮细胞出芽的活性（P〈0.05）。结论麝香保心丸及人参皂苷Rg3、Rh2均在体外具有促进血管新生的活性。Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill（ SBP） presenting in the plasma. Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell proliferation and cell migration assays by xCELLigence. And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect. Results SBP（ 10^- 4- 10^- 2μg/ml）,ginsenoside Rg3（ 1 ~ 10 μmol/L） and ginsenoside Rh2（ 1 - 10μmol/L） significantly stimulated human umbilical vein endothelial cells（ HUVECs） proliferation,migration and tube-like structures formation at different concentrations（ P〈0. 05）. In addition,compared to the control group,only the high concentration group of SBP（ 10- 2μg/ml）,Rg3（ 10 μmol/L） and Rh2（ 10 μmol/L） could induce endothelial cell sprouting from the aortic ring（ P〈0. 05）. Conclusion SBP,ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.

关 键 词：麝香保心丸 人参皂苷RG3 人参皂苷RH2 血管新生 

分 类 号：R972[医药卫生—药品]