蒽酮染色法测定维地红涂剂中多糖的含量  

Determination of the polysaccharide in Verdihong paint

作　　者：余学英[1] 常明泉[1] 陈芳[1] 王刚[1] 

机构地区：[1]湖北医药学院附属太和医院,湖北十堰442000

出　　处：《药学实践杂志》2014年第5期352-353,356,共3页Journal of Pharmaceutical Practice

基　　金：太和科研基金资助项目(2009D34)

摘　　要：目的建立维地红涂剂中多糖的含量测定方法。方法用无水葡萄糖为对照品,0.2%蒽酮-硫酸试液为染色剂,紫外分光光度法测定,测定波长620 nm。结果多糖在1.9-34.2μg/ml范围内线性关系良好,Y=0.033X-0.006 7（r=0.999 9）,回收率为98.6%,RSD=1.06%（n=6）。结论所建方法便捷、重现性好,适用于维地红涂剂中多糖的含量测定。Objective To establish the determination method of the polysaccharide in Verdihong paint. Methods The anhydrous dextrose was used as the control,0. 2% anthrone-sulfuric acid as the stain,the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm. Results The polysaccharide curve was linear within a range of 1. 9 ~ 34. 2μg/ml,linear equation was Y =0. 033 X- 0. 006 7（ r = 0. 999 9）,the average recovery was 98. 6% with RSD of 1. 06%（ n = 6）.Conclusion This method was convenient,stable and coud be used to assay content of the polysaccharide in Verdihong paint.

关 键 词：维地红涂剂 多糖 含量测定 

分 类 号：R927.2[医药卫生—药学]